Ammonia assimilation is crucial in microbial nitrogen metabolism, and researching the impact of magnetic field (MF) on heterotrophic ammonia assimilation (HAA) contributes to improving nitrogen utilization and environmental remediation. This study systematically investigated the profound effects of MF stimulation on carbon and ammonia assimilation mechanisms in heterotrophic microorganisms. The dynamic responses of microbial carbon source metabolic efficiency and nitrogen source assimilation rates were quantitatively analyzed by designing a multidimensional stimulation environment of different nutrient levels (C/N 20, 25, 30) and MF intensities (0, 1, 20 mT). The findings demonstrated that weak MF stimulation markedly enhanced carbon utilization efficiency and ammonia assimilation capacity, evidenced by accelerated metabolic flux, significant biomass augmentation and metabolic network reconfiguration. MF induced pronounced activation of key metabolic enzymes, uncovering coordinated regulatory mechanisms at the molecular level. Additionally, the MF stimulation enriched genera the Halomonas and Marinobacter, enhancing the stability of microbial community. These discoveries not only advance the theoretical understanding of MF-enhanced metabolic modulation but also offer cutting-edge insights and potential solutions for developing MF-assisted bioconversion and environmental remediation technologies.
Keywords: Carbon utilization efficiency; Heterotrophic ammonia assimilation; Magnetic field; Microbial network.
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