Polystyrene nanoparticles pose significant toxicological risks to aquatic ecosystems, yet their impact on zebrafish ( Danio rerio) embryonic development, particularly erythropoiesis, remains underexplored. This study used single-cell RNA sequencing to comprehensively evaluate the effects of polystyrene nanoparticle exposure on erythropoiesis in zebrafish embryos. In vivo validation experiments corroborated the transcriptomic findings, revealing that polystyrene nanoparticle exposure disrupted erythrocyte differentiation, as evidenced by the decrease in mature erythrocytes and concomitant increase in immature erythrocytes. Additionally, impaired heme synthesis further contributed to the diminished erythrocyte population. These findings underscore the toxic effects of polystyrene nanoparticles on hematopoietic processes, highlighting their potential to compromise organismal health in aquatic environments.
聚苯乙烯纳米颗粒对水生生态系统构成重要毒理风险，然而它们对斑马鱼（ Danio rerio）胚胎发育，特别是红细胞生成的影响尚未得到充分探讨。该研究利用单细胞RNA测序全面评估了聚苯乙烯纳米颗粒暴露对斑马鱼胚胎红细胞生成的影响。体内验证实验证实了转录组学发现，揭示聚苯乙烯纳米颗粒暴露扰乱了红细胞分化，体现为成熟红细胞减少和不成熟红细胞增加。此外，受损的血红素合成进一步导致了红细胞数量的减少。上述结果表明了聚苯乙烯纳米颗粒对造血过程的毒性影响，它们可能损害水生环境中生物健康的潜力。.
Keywords: Erythropoiesis; Polystyrene nanoparticles; Single-cell RNA sequencing; Zebrafish embryos.