Effect of high-fat diet on IgA+ cells and BAFF/APRIL in small intestinal villous lamina propria of mice

Yuta Sakamoto; Masatoshi Niwa; Ken Muramatsu; Satoshi Shimo

doi:10.1016/j.cellimm.2024.104911

Effect of high-fat diet on IgA⁺ cells and BAFF/APRIL in small intestinal villous lamina propria of mice

Cell Immunol. 2025 Jan 21:409-410:104911. doi: 10.1016/j.cellimm.2024.104911. Online ahead of print.

Authors

Yuta Sakamoto¹, Masatoshi Niwa², Ken Muramatsu³, Satoshi Shimo⁴

Affiliations

¹ Faculty of Health Sciences, Department of Rehabilitation, Health Science University, 7187 Kodachi, Fujikawaguchiko-Machi, Minamitsuru-Gun, Yamanashi, Japan; Graduate School of Health Sciences, Kyorin University, 5-4-1 Shimorenjaku, Mitaka-shi, Tokyo, Japan. Electronic address: y.sakamoto@kenkoudai.ac.jp.
² Faculty of Health Sciences, Department of Rehabilitation, Kyorin University, 5-4-1 Shimorenjaku, Mitaka-shi, Tokyo, Japan. Electronic address: mt-niwa@ks.kyorin-u.ac.jp.
³ Faculty of Health Sciences, Department of Rehabilitation, Kyorin University, 5-4-1 Shimorenjaku, Mitaka-shi, Tokyo, Japan. Electronic address: k-muramatsu@ks.kyorin-u.ac.jp.
⁴ Faculty of Health Sciences, Department of Rehabilitation, Health Science University, 7187 Kodachi, Fujikawaguchiko-Machi, Minamitsuru-Gun, Yamanashi, Japan. Electronic address: sshimo@kenkoudai.ac.jp.

PMID: 39842230
DOI: 10.1016/j.cellimm.2024.104911

Abstract

Obesity exacerbates susceptibility to infectious diseases. We investigated the effects of a high-fat diet (HFD) on intestinal immunity, particularly immunoglobulin (Ig)A-producing cells, B-cell activating factor (BAFF), and a proliferation-inducing ligand (APRIL) localization. Mice (4- to 20-weeks old) were fed HFD or standard chow diet, and their jejunum and ileum were fixed using the in vivo cryotechnique. Immunohistochemistry was performed for IgA, BAFF, and APRIL. In the HFD group, IgA⁺, IgA⁺CD22⁺ (p < 0.001), and IgA⁺CD138^- (p = 0.007) cell counts were diminished in the middle sections of the lamina propria of jejunal villi, and BAFF levels were significantly reduced in jejunal villi. The HFD effects on IgA⁺ cell distribution seem to be confined to jejunal villi, hinting at localized vulnerabilities in intestinal immunity during obesity. Moreover, in the HFD group, IgA⁺ B-cell counts were reduced in the middle jejunum, indicating inhibition of the IgA⁺ B-cells through a T-cell-independent pathway.

Keywords: High-fat feeding; Immunoglobulin-a-positive cell; In vivo cryotechnique; Intestinal immunity; T-cell-independent pathway.