Chromosome-level genome assembly of the pine wood nematode carrier Arhopalus unicolor

Xiangying Zou; Liqiong Zeng; Xingzao You; Jinghui Zhong; Chensheng Tang; Zhaofei Zheng; Xiaochun Lin; Biaoqiang Zhang; Xinliang Zhang; Yu Fang; Yanli Sun; Shouping Cai; Jun Su

doi:10.1038/s41597-025-04460-8

Chromosome-level genome assembly of the pine wood nematode carrier Arhopalus unicolor

Sci Data. 2025 Jan 20;12(1):111. doi: 10.1038/s41597-025-04460-8.

Authors

Xiangying Zou^#^{1

2}, Liqiong Zeng^#¹, Xingzao You³, Jinghui Zhong⁴, Chensheng Tang¹, Zhaofei Zheng⁵, Xiaochun Lin⁶, Biaoqiang Zhang⁷, Xinliang Zhang⁸, Yu Fang⁹, Yanli Sun¹⁰, Shouping Cai¹¹, Jun Su¹²

Affiliations

¹ Key Laboratory of State Forestry and Grassland Administration on Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China, Fujian Academy of Forestry, Fuzhou, 350012, China.
² Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, College of Life Sciences, Fuzhou, 350002, China.
³ Nanping Forestry Science and Technology Promotion Center, Nanping, 354200, China.
⁴ Quanzhou Forestry Pest Control and Quarantine Station, Quanzhou, 362000, China.
⁵ Nanping Forestry Bureau, Nanping, 353000, China.
⁶ Fuding Forestry Bureau, Fuding, 355200, China.
⁷ Shaxian Guanzhuang State-owned Forest Farm of Fujian Province, Shaxian, 365050, China.
⁸ Shaxian Forestry Pest Control and Quarantine Station, Shaxian, 365500, China.
⁹ Institute of Resources, Environment and Soil Fertilizer, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, China.
¹⁰ Lanshan Forestry Development Centre, Linyi, 276001, China.
¹¹ Key Laboratory of State Forestry and Grassland Administration on Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China, Fujian Academy of Forestry, Fuzhou, 350012, China. caishouping@163.com.
¹² Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, College of Life Sciences, Fuzhou, 350002, China. junsu@fafu.edu.cn.

^# Contributed equally.

Abstract

Arhopalus unicolor is a carrier of the pine wood nematode (PWN), which causes pine wilt disease, killing pine trees and causing considerable economic and environmental losses. While the A. unicolor mitochondrial genome has been published, a high-quality genome assembly and annotation of A. unicolor is not yet available. To address this, we assembled a chromosome-level reference genome assembly of A. unicolor with a combination of Illumina, PacBio, and Hi-C sequencing technologies. The final genome size was determined to be 1268.11 Mb, with a GC% of 32.44%, and the scaffold N50 value was 19.30 Mb. A total of 98.77% of the assembled sequences mapped to 10 pseudochromosomes, and BUSCO analysis revealed high completeness, with 97.15% gene coverage. Furthermore, the genome contains 71.74% repeat elements and encompasses 16,450 predicted protein-coding genes. This genome sequence of A. unicolor will be a valuable resource for understanding the genetics and evolutionary history of this species and for developing effective management strategies for this PWN carrier.

Publication types

Dataset

MeSH terms

Animals
Chromosomes
Genome Size
Genome, Helminth
Genome, Mitochondrial
Pinus* / parasitology
Plant Diseases / parasitology
Tylenchida / genetics