Systematic Molecular Influenza A/B Screening Upon Hospital Admission in Belgium, January-April 2022: Positivity Ratios and Viral Loads According to Symptomatology, Age, and Vaccination Status

J Med Virol. 2025 Jan;97(1):e70167. doi: 10.1002/jmv.70167.

Abstract

Three hospitals implemented molecular point-of-care tests (POCTs) to screen patients for SARS-CoV-2 infection upon admission during the 2021/2022 influenza season, which in Belgium lasted from January to April 2022. The samples were simultaneously tested for influenza A/B. Influenza positivity at admission was examined in relation to patient characteristics and symptomatology. Influenza POCTs were performed on all patients requiring urgent hospitalization, regardless of the admission reason. A total of 9327 patients were included in the study, of which 411 (4.4%) tested positive for influenza A/B. Asymptomatic infection and mild illness accounted for respectively 11.2% (95% CI: 8.5%-14.6%), and 43.3% (95% CI: 38.6%-48.1%) of the cases. A total of 66% (95% CI: 60%-72%) of all patients in these symptom categories (asymptomatic and mild illness) showed a high viral load (cycle threshold [Ct] < 24). Only in 30 (7.3%, 95% CI: 5.2%-10.2%) of all cases and in two (4.4%, 95% CI: 1.2%-14.5%) of the asymptomatic cases, the symptomatology worsened during hospital stay. Coinfections with both influenza and SARS-CoV-2 occurred in 35 patients (8.5% of all influenza positive patients). There was no difference in symptomatology between patients with co-infections and those with an influenza mono-infection. Patients could not be reliably categorized into carriers with low versus high viral loads based on symptomatology, age, and vaccination status. More than half of the influenza-positive individuals were either asymptomatic or had mild symptoms upon admission, while often carrying high viral loads. Our results show that without screening of patients at hospital admission, a considerable number of patients with a high viral load may be incorrectly classified as being not infectious.

Keywords: LIAT; POCT; epidemiology; influenza.

MeSH terms

  • Adolescent
  • Adult
  • Age Factors
  • Aged
  • Aged, 80 and over
  • Belgium / epidemiology
  • COVID-19* / diagnosis
  • COVID-19* / epidemiology
  • COVID-19* / virology
  • Child
  • Child, Preschool
  • Female
  • Hospitalization* / statistics & numerical data
  • Humans
  • Infant
  • Influenza A virus* / genetics
  • Influenza A virus* / isolation & purification
  • Influenza B virus* / genetics
  • Influenza B virus* / isolation & purification
  • Influenza, Human* / diagnosis
  • Influenza, Human* / epidemiology
  • Influenza, Human* / virology
  • Male
  • Mass Screening / methods
  • Middle Aged
  • Point-of-Care Testing
  • SARS-CoV-2* / genetics
  • SARS-CoV-2* / isolation & purification
  • Vaccination / statistics & numerical data
  • Viral Load*
  • Young Adult