[A new nortriterpenoid from mastic]

Zhongguo Zhong Yao Za Zhi. 2024 Dec;49(23):6342-6351. doi: 10.19540/j.cnki.cjcmm.20240910.202.
[Article in Chinese]

Abstract

Ten compounds were separated by various modern chromatographic methods from mastic. They were identified by HR-ESI-MS, IR, UV, NMR, quantum chemical calculation of NMR(qcc-NMR) and comparison with reported data in literature as 17β-hydroxy-28-norolean-18-en-3-one(1), 28-norolean-12,17-dien-3,11-dione(2), 28-norolean-16,18-dien-3-one(3),(24Z)-26-hydroxy-7,24-dientirucalla-3-one(4), masticadienonic acid(5)、masticadienolic acid(6)、erythrodiol(7), 3β,28-dihydroxyoleana-11,13(18)-diene(8), 3-oxo-olean-9(11),12-dien-28-oic acid(9), and 12-oleane-3,11-dione(10). Among them, compound 1 was a novel compound and compound 2 was a novel natural product. Compounds 1-2, 4, and 7-9 were isolated from mastic for the first time. Compound 1 significantly inhibited lipopolysaccharide-induced production of nitric oxide in mouse monocyte macrophage RAW264.7 cells with the IC_(50) of(7.44±0.49) μmol·L~(-1), which was more potent than the positive control, dexamethasone \[IC_(50)=(9.93±1.17) μmol·L~(-1)\]. Compounds 2-3, 5, and 9-10 also showed inhibitory effects, with the IC_(50) ranging from 14.82 to 28.82 μmol·L~(-1). Compounds 4-5, and 7 markedly inhibited the growth of human colon adenocarcinoma cells SW480, with the IC_(50) of(16.13±1.69),(27.40±1.81), and(10.01±0.10) μmol·L~(-1), respectively.

Keywords: anti-colorectal cancer; anti-inflammatory; chemical constituents; mastic; nortriterpenoid.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Drugs, Chinese Herbal / chemistry
  • Drugs, Chinese Herbal / pharmacology
  • Humans
  • Macrophages / drug effects
  • Magnetic Resonance Spectroscopy
  • Mastic Resin / chemistry
  • Mice
  • Molecular Structure
  • Nitric Oxide
  • RAW 264.7 Cells
  • Triterpenes* / chemistry
  • Triterpenes* / pharmacology

Substances

  • Triterpenes
  • Mastic Resin
  • Nitric Oxide
  • Drugs, Chinese Herbal