Evaluation of antibacterial and antifungal activities of a commercially available herbal mouthwash in accordance with the PN-EN 1040 and EN 1650 standards

Ram Sabarish; Raja Lakshmi A; Reshma Achu Joseph; Kennedy Kumar Palraj; Prema Malini Thayanidhi; Muthukumar Santhanakrishnan

doi:10.1016/j.diagmicrobio.2025.116680

Evaluation of antibacterial and antifungal activities of a commercially available herbal mouthwash in accordance with the PN-EN 1040 and EN 1650 standards

Diagn Microbiol Infect Dis. 2025 Jan 9;111(3):116680. doi: 10.1016/j.diagmicrobio.2025.116680. Online ahead of print.

Authors

Ram Sabarish¹, Raja Lakshmi A², Reshma Achu Joseph³, Kennedy Kumar Palraj⁴, Prema Malini Thayanidhi⁴, Muthukumar Santhanakrishnan²

Affiliations

¹ Department of Periodontology & Implantology, Sri Ramachandra Dental College and Hospital, Sri Ramachandra Institute of Higher Education and Research (DU), Address: No:1, Ramachandra Nagar,porur, Chennai 600116, India. Electronic address: ramsabarish@sriramachandra.edu.in.
² Department of Periodontology & Implantology, Sri Ramachandra Dental College and Hospital, Sri Ramachandra Institute of Higher Education and Research (DU), Address: No:1, Ramachandra Nagar,porur, Chennai 600116, India.
³ Department of Periodontology & Implantology, Ragas Dental College & Hospital, The Tamil Nadu Dr M.G.R Medical University, Uthandi, Tamil Nadu 600119, India.
⁴ Department of Microbiology, Sri Ramachandra Medical College and Research Institute, Sri Ramachandra Institute of Higher Education and Research (DU), Address: No:1, Ramachandra Nagar, Porur, Chennai 600116, India.

PMID: 39805232
DOI: 10.1016/j.diagmicrobio.2025.116680

Abstract

Periodontitis is complex microbial plaque induced condition. In this study, HiOra herbal mouthwash widely marketed in India, achieved a 5-log reduction in standard fungi and bacterial strains within 60 min. Thereby demonstrating antibacterial and antifungal activity similar to chlorhexidine and accordance with the PN EN 1040 and EN 1650 standards.

Keywords: Antimicrobial resistance; Chemical disinfectant; Chlorohexidine; Herbal mouthwash.