Biophysical interactions between self-sufficient cytochrome P450 from Tepidiphilus thermophilus and ilaprazole

Dalton Trans. 2025 Jan 21;54(4):1354-1359. doi: 10.1039/d4dt03320e.

Abstract

Proton pump inhibitors (PPIs), metabolized by cytochrome P450 (P450) enzymes, are widely used to inhibit gastric acid secretion. This study investigated CYP116B46, a self-sufficient monooxygenase with a reductase domain, to elucidate its interaction with ilaprazole, a PPI. Binding assays and docking simulations indicate that CYP116B46 serves as a suitable model for studying PPI metabolism.

MeSH terms

  • 2-Pyridinylmethylsulfinylbenzimidazoles / chemistry
  • 2-Pyridinylmethylsulfinylbenzimidazoles / metabolism
  • Cytochrome P-450 Enzyme System* / chemistry
  • Cytochrome P-450 Enzyme System* / metabolism
  • Molecular Docking Simulation*
  • Protein Binding
  • Proton Pump Inhibitors / chemistry
  • Proton Pump Inhibitors / metabolism
  • Proton Pump Inhibitors / pharmacology

Substances

  • Cytochrome P-450 Enzyme System
  • ilaprazole
  • Proton Pump Inhibitors
  • 2-Pyridinylmethylsulfinylbenzimidazoles