In recent years, the incidence of gastric cancer (GC) has been on the rise, surgical procedures usually require the removal of part of gastric tissue connected with the tumor lesion, which leads to poor postoperative health and adverse prognosis in patients. Probiotics, as an active microorganism, play an important role in improving gastrointestinal function and enhancing immunity. In this study, we randomized 135 GC patients into a control group, a probiotic group and a combination group. All the study subjects were treated with radical GC resection in our hospital. Postoperatively, the control group was given routine treatment, the probiotic group received routine treatment + probiotics and the combined group was given routine treatment+ probiotics +Shenqi injection. Through research, we found that after the use of probiotics, the postoperative rehabilitation process of GC patients was significantly shortened compared to patients treated with conventional enteral nutrition suspension, the immune function and nutritional status were validly improved and the prognostic survival rate was increased. In addition, the CD4+, ALB, HGB, PA and TP of patients in the combination group were higher than those in the probiotic group (P<0.05), while CD4+/CD8+ were lower than those in the probiotic group (P<0.05).