Characterization of fungal carbonyl sulfide hydrolase belonging to clade D β-carbonic anhydrase

Ryuka Iizuka; Takahiro Ogawa; Rikako Tsukida; Keiichi Noguchi; David Hibbett; Yoko Katayama; Makoto Yoshida

doi:10.1002/1873-3468.15098

Characterization of fungal carbonyl sulfide hydrolase belonging to clade D β-carbonic anhydrase

FEBS Lett. 2025 Jan 10. doi: 10.1002/1873-3468.15098. Online ahead of print.

Authors

Ryuka Iizuka¹, Takahiro Ogawa², Rikako Tsukida³, Keiichi Noguchi⁴, David Hibbett⁵, Yoko Katayama^{3

6}, Makoto Yoshida^{1

3

7}

Affiliations

¹ Department of Symbiotic Science of Environment and Natural Resources, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Japan.
² Graduate School of Engineering, Tokyo University of Agriculture and Technology, Koganei, Japan.
³ Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Japan.
⁴ Instrumentation Analysis Center, Tokyo University of Agriculture and Technology, Koganei, Japan.
⁵ Department of Biology, Clark University, Worcester, MA, USA.
⁶ Independent Administrative Institution, Tokyo National Research Institute for Cultural Properties, Taito-ku, Japan.
⁷ Institute of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Japan.

PMID: 39792592
DOI: 10.1002/1873-3468.15098

Abstract

Carbonyl sulfide hydrolase (COSase) is a unique enzyme that exhibits high activity towards carbonyl sulfide (COS) but low carbonic anhydrase (CA) activity, despite belonging to the CA family. COSase was initially identified in a sulfur-oxidizing bacterium and later discovered in the ascomycete Trichoderma harzianum strain THIF08. The COSase from T. harzianum has been recognized as a key enzyme in the assimilation of gaseous COS, and homologous genes are widely present not only in Ascomycota but also in Basidiomycota. Here, we characterized the COSases from the basidiomycete Gloeophyllum trabeum NBRC 6430 and T. harzianum to obtain detailed characteristics of fungal COSase. This study contributes to a better understanding of COS metabolism in fungi.

Keywords: carbonic anhydrase; carbonyl sulfide; carbonyl sulfide hydrolase; fungi; sulfur metabolism.

Abstract

Grants and funding