Amino acid-based, sustainable organic nanozyme and integrated sensing platform for histamine detection

Dong Hoon Lee; Mohammed Kamruzzaman

doi:10.1016/j.foodchem.2025.142751

Amino acid-based, sustainable organic nanozyme and integrated sensing platform for histamine detection

Food Chem. 2025 Jan 4:471:142751. doi: 10.1016/j.foodchem.2025.142751. Online ahead of print.

Authors

Dong Hoon Lee¹, Mohammed Kamruzzaman²

Affiliations

¹ The Grainger College of Engineering, College of Agricultural, Consumer and Environmental Sciences, Department of Agricultural and Biological Engineering, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA.
² The Grainger College of Engineering, College of Agricultural, Consumer and Environmental Sciences, Department of Agricultural and Biological Engineering, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA. Electronic address: mkamruz1@illinois.edu.

PMID: 39788018
DOI: 10.1016/j.foodchem.2025.142751

Abstract

Inorganic nanozymes hold promise for biomolecule sensing but face challenges like complex fabrication, toxicity, and low sustainability, limiting their use. To overcome these, a sustainable organic nanozyme (OA nanozyme) was created using amino acids and a biocompatible polymer for effective histamine detection. The OA nanozyme exhibits peroxidase-like activity and was fabricated through a single chelation/polymer entanglement method, enabling rapid production (within 3 h) with uniform morphology (≤100 nm diameter) and a negative surface charge at neutral pH. It shows decent kinetic performance (Km = 0.009 mM for H₂O₂) and biocompatibility, making it suitable for biological applications. The OA nanozyme achieved high sensitivity for histamine detection (LOD: 21.37 pgmL^-1) with excellent selectivity and specificity against related molecules. The system's ability to detect histamine in real food samples (e.g., spinach) was also confirmed. These findings indicate that the OA nanozyme holds significant potential for broader applications in food safety and quality assurance.

Keywords: Amino acid-based organic nanozyme; Colorimetric sensor; Histamine detection; Peroxidase-like activity; Sustainable nanozyme.