Autotrophic ammonium nitrogen removal process mediated by manganese oxides: Bioreactors performance optimization and potential mechanisms

Yue Wang; Yihan Bai; Liang Xu; Junfeng Su; Miqi Ren; Chenxi Hou; Jingting Feng

doi:10.1016/j.envres.2025.120778

Autotrophic ammonium nitrogen removal process mediated by manganese oxides: Bioreactors performance optimization and potential mechanisms

Environ Res. 2025 Jan 5:268:120778. doi: 10.1016/j.envres.2025.120778. Online ahead of print.

Authors

Yue Wang¹, Yihan Bai¹, Liang Xu¹, Junfeng Su², Miqi Ren¹, Chenxi Hou¹, Jingting Feng¹

Affiliations

¹ School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
² School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China. Electronic address: sjf1977518@sina.com.

PMID: 39765308
DOI: 10.1016/j.envres.2025.120778

Abstract

Manganese(IV) (Mn(IV)) reduction coupled with ammonium (NH₄⁺-N) oxidation (Mnammox) has been found to play a significant role in the nitrogen (N) cycle within natural ecosystems. However, research and application of the autotrophic NH₄⁺-N removal process mediated by manganese oxides (MnOx) in wastewater treatment are currently limited. This study established autotrophic NH₄⁺-N removal sludge reactors mediated by various MnOx types, including δ-MnO₂ (δ-MSR), β-MnO₂ (β-MSR), α-MnO₂ (α-MSR), and natural Mn ore (MOSR), investigating their NH₄⁺-N removal performances and mechanisms under different initial N loading and pH conditions. During the 330 d operation, the reactors exhibited NH₄⁺-N removal efficiencies in the order of δ-MSR > α-MSR > β-MSR > MOSR. Notably, metal-reducing bacteria (Candidatus Brocadia, Dechloromonas, and Rhodocyclaceae) and Mn(II) oxidizing bacteria (Pseudomonas and Zoogloea) were enriched in the reactors, especially in the δ-MSR. The presence of these microorganisms facilitated the reduction of Mn(IV) and utilized the generated Mn(II) to drive autotrophic denitrification (MnOAD), thereby completing the Mn(IV)/Mn(II) cycle and enhancing N removal in the system. An active Mn cycle displayed in δ-MSR, which could be demonstrated by the formation of petal-shaped biogenic MnOx and the increased abundance of Mn cycling genes (MtrCDE, MtrA, MtrB, and CotA, etc.). Meanwhile, genes involved in N metabolism were enriched, particularly functional genes associated with nitrification and denitrification. In this study, the coupling of Mnammox and MnOAD was realized via the Mn cycle, providing a new perspective on the application of autotrophic N removal technologies in wastewater treatment.

Keywords: Ammonium removal; Manganese cycle; Manganese oxidation; Manganese oxide; MnOx driving ammonium oxidation.