Detection of amino thiols from fish samples by stable isotope chemical labelling coupled with ultra-performance liquid chromatography-tandem mass spectrometry

Siyuan Wu; Yang Feng; Shengjun Chen; Yongqiang Zhao; Jianchao Deng; Di Wang

doi:10.1016/j.foodchem.2024.142728

Detection of amino thiols from fish samples by stable isotope chemical labelling coupled with ultra-performance liquid chromatography-tandem mass spectrometry

Food Chem. 2024 Dec 31:471:142728. doi: 10.1016/j.foodchem.2024.142728. Online ahead of print.

Authors

Siyuan Wu¹, Yang Feng², Shengjun Chen³, Yongqiang Zhao⁴, Jianchao Deng⁴, Di Wang⁵

Affiliations

¹ Key Laboratory of Aquatic product, Ministry of Agriculture and Rural affairs, National R&D Center for Aquatic Product Processing, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510330, China; College of Food Science, Shanghai Ocean University, Shanghai 201306, China.
² Key Laboratory of Aquatic product, Ministry of Agriculture and Rural affairs, National R&D Center for Aquatic Product Processing, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510330, China; Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya Tropical Fisheries Research Institute, Sanya 572426, China. Electronic address: fengyang@scsfri.ac.cn.
³ Key Laboratory of Aquatic product, Ministry of Agriculture and Rural affairs, National R&D Center for Aquatic Product Processing, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510330, China; Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya Tropical Fisheries Research Institute, Sanya 572426, China; College of Food Science, Shanghai Ocean University, Shanghai 201306, China. Electronic address: chenshengjun@scsfri.ac.cn.
⁴ Key Laboratory of Aquatic product, Ministry of Agriculture and Rural affairs, National R&D Center for Aquatic Product Processing, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510330, China; Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya Tropical Fisheries Research Institute, Sanya 572426, China; College of Food Science, Shanghai Ocean University, Shanghai 201306, China.
⁵ Key Laboratory of Aquatic product, Ministry of Agriculture and Rural affairs, National R&D Center for Aquatic Product Processing, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510330, China; Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya Tropical Fisheries Research Institute, Sanya 572426, China.

PMID: 39764942
DOI: 10.1016/j.foodchem.2024.142728

Abstract

The amino thiols are key antioxidants in organisms, and their detection in food is of significant importance. This study developed a new stable isotope chemical labelling coupled with ultra-performance liquid chromatography-tandem mass spectrometry method to detect six amino thiols from fish samples. By the proposed method, amino thiols were labeled after liquid extraction using the stable isotope labeling reagents of iodoacetamide (IAM) and D₄-IAM. Desirable recovery and linearity of thiols were achieved in fish samples. The limits of detection (LOD) ranged from 0.2 to 6.5 nmol/g, and the detection sensitivity was improved from 2.6 to 74 folds. The cluster analysis revealed that this method successfully discriminated eight fish species and their sources. Additionally, we found that glutathione and cysteine were significantly correlated with the transportation stress status of golden pompano (Trachinotus ovatus). Overall, this study presents a sensitive and efficient method for the detection of amino thiols from fish samples.

Keywords: Amino thiols; Iodoacetamide; Liquid extraction; Stable isotope labeling; Transportation stress; UPLC-MS.