A novel lncRNA MSTRG.59348.1 regulates muscle cells proliferation and innate immunity of Megalobrama amblycephala

Qianhui Sun; Wenya Zhai; Huanling Wang; Zexia Gao; Hong Liu

doi:10.1016/j.ijbiomac.2024.139445

A novel lncRNA MSTRG.59348.1 regulates muscle cells proliferation and innate immunity of Megalobrama amblycephala

Int J Biol Macromol. 2025 Jan 3:139445. doi: 10.1016/j.ijbiomac.2024.139445. Online ahead of print.

Authors

Qianhui Sun¹, Wenya Zhai², Huanling Wang², Zexia Gao², Hong Liu³

Affiliations

¹ College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affair, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, China; Xiaogan Academy of Agricultural Sciences, Xiaogan 432100, China.
² College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affair, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, China.
³ College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affair, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, China. Electronic address: liuhong59@mail.hzau.edu.cn.

PMID: 39756731
DOI: 10.1016/j.ijbiomac.2024.139445

Abstract

In mammals, long non-coding RNAs (lncRNAs) play a regulatory role in gene expression, contribute to immune responses, and aid in pathogen elimination, primarily through interactions with RNA-binding proteins (RBPs). However, the role of lncRNAs in fish innate immunity and their interaction with RBPs remains uncertain. To investigate the immunomodulatory role of lncRNAs in Megalobrama amblycephala, we identified the novel lncRNA MSTRG.59348.1 and examined its function in the innate immune response to Aeromonas hydrophila infection. Localization studies in hepatocytes revealed that MSTRG.59348.1 is primarily located in the nucleus, suggesting its potential involvement in gene regulation, possibly through chromatin modification or other nuclear processes. The expression of MSTRG.59348.1 was significantly up-regulated after lipopolysaccharide (LPS) stimulation in liver cells. RNA-seq analysis of muscle cells revealed that genes differentially expressed following MSTRG.59348.1 overexpression were enriched in immune pathways. MSTRG.59348.1 overexpression significantly inhibited the expression of sting and ifn, and significantly up-regulated muscle cell viability and promoted cell proliferation by targeting sting, ifn, nf-κb1, and bcl2. Screening by RNA pull-down and mass spectrometry identified 57 RBPs interacting with MSTRG.59348.1, with functions enriched in immune pathways. Our results suggest that MSTRG.59348.1 plays a crucial regulatory role in fish antibacterial response, marking it as a significant subject for future research in innate immunity.

Keywords: Cells proliferation; Innate immunity; Megalobrama amblycephala; RNA-binding proteins; lncRNA.