In Vivo Clonal Analysis Using MADM with Spatiotemporal Specificity

Methods Mol Biol. 2025:2886:265-279. doi: 10.1007/978-1-0716-4310-5_13.

Abstract

Mosaic analysis with double markers (MADM) is a powerful in vivo lineage tracing technique. It utilizes Cre recombinase-dependent interchromosomal recombination to restore the stable expression of two fluorescent proteins sparsely in individual dividing stem or progenitor cells and their progenies. Here, we describe the application of this technique for quantitative lineage analysis of radial glial progenitors in the developing mouse neocortex at the single-cell resolution.

Keywords: 3D reconstruction; Clonal analysis; Interchromosomal recombination; Lineage tracing; MADM; Serial sectioning.

MeSH terms

  • Animals
  • Cell Lineage* / genetics
  • Ependymoglial Cells / cytology
  • Ependymoglial Cells / metabolism
  • Integrases* / genetics
  • Integrases* / metabolism
  • Mice
  • Mosaicism
  • Neocortex / cytology
  • Neocortex / growth & development
  • Neocortex / metabolism
  • Recombination, Genetic
  • Single-Cell Analysis / methods

Substances

  • Cre recombinase
  • Integrases