Operating principles of interconnected feedback loops driving cell fate transitions

NPJ Syst Biol Appl. 2025 Jan 2;11(1):2. doi: 10.1038/s41540-024-00483-w.

Abstract

Interconnected feedback loops are prevalent across biological mechanisms, including cell fate transitions enabled by epigenetic mechanisms in carcinomas. However, the operating principles of these networks remain largely unexplored. Here, we identify numerous interconnected feedback loops implicated in cell lineage decisions, which we discover to be the hallmarks of lower- and higher-dimensional state space. We demonstrate that networks having higher centrality nodes have restricted state space while those with lower centrality nodes have higher dimensional state space. The topologically distinct networks with identical node or loop counts have different steady-state distributions, highlighting the crucial influence of network structure on emergent dynamics. Further, regardless of topology, networks with autoregulated nodes exhibit multiple steady states, thereby "liberating" network dynamics from absolute topological control. These findings unravel the design principles of multistable networks implicated in fate decisions and can have crucial implications in engineering or comprehending multi-fate decision circuits.

MeSH terms

  • Cell Differentiation* / genetics
  • Cell Differentiation* / physiology
  • Cell Lineage* / genetics
  • Feedback, Physiological / physiology
  • Gene Regulatory Networks / genetics
  • Humans
  • Models, Biological
  • Systems Biology / methods