Efficacy determination of a disinfectant against channel catfish virus by in vitro and in vivo methods

In Vitro Cell Dev Biol Anim. 2024 Dec 30. doi: 10.1007/s11626-024-01003-0. Online ahead of print.

Abstract

Channel catfish virus (CCV) poses a significant threat to catfish culture. Lack of effective vaccines and antiviral treatments necessitates effective disinfection strategies to mitigate its spread. In vitro trials indicated the virus to be inactivated at high temperatures, but was infectious at 40°C. This study evaluated the efficacy of a commercial disinfectant against CCV using both in vitro and in vivo approaches. In vitro experiments assessed the virucidal activity of the disinfectant against CCV in channel catfish ovary (CCO) cells, while in vivo trials evaluated its effectiveness in reducing viral transmission and mortality among channel and hybrid catfish fingerlings. Results indicated that the disinfectant was effective in inactivating the virus at the tested concentrations and improved the survival of fish exposed to the virus. This study provides critical insights into selecting appropriate disinfection protocols to enhance biosecurity in catfish hatchery settings and to mitigate CCV transmission.

Keywords: Aquaculture; Channel catfish virus; Disinfectant; Efficacy; Inactivation.