A Method for the Separation of Phytophthora Oosporesfrom Soil for DNA-Based Detection

Abstract

Here, we present a protocol for the isolation and detection of Phytophthora oospores directly from soil samples. Our method incorporates a novel technique for isolating Phytophthora oospores using filter pouches and an improved DNA extraction procedure specifically designed for oospores. While we have primarily developed this protocol for detecting P. agathidicida oospores using end-point PCR, we believe these methods can be readily adapted for other Phytophthora species. Furthermore, the DNA extracted using this protocol is suitable as input for other DNA-based detection methods.

Keywords: DNA; Detection; Filtration; Oospores; PCR; Phytophthora agathidicida; Soil.