Accurately monitoring the content of Cu2+ and Hg2+ in real samples and biological systems is of great significance in ensuring food safety and human health. Therefore, developing efficient methods for simultaneously detecting Cu2+ and Hg2+ is of great significance in living organisms and real samples. In this work, a bifunctional ratiometric and colorimetric fluorescent bio-sensor DPT-1, was designed and synthesized using triphenylamine to specifically and concurrently detect Cu2+ and Hg2+ exhibited varying degrees of fluorescence quenching through "turn-off" fluorescence. Furthermore, DPT-1 effectively detected Cu2+ and Hg2+ in various real samples, encompassing tap water, Songhua River water, and honeysuckle extract. In addition, DPT-1 exhibited remarkable performance in detecting Cu2+/Hg2+ in rice roots, living cells and zebrafish. As research progressed, the discoveries outlined in this article offered the versatile potential for holding promise for food safety monitoring and biological research.
Keywords: Fluorescent bio-sensor; Heavy metal Cu(2+)/Hg(2+) detection; Laser confocal imaging; Ratiometric and colorimetric sensor.
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