Sodium cholate-coated Olea europaea polyphenol nanoliposomes: Preparation, stability, release, and bioactivity

Wenjun Li; Lemonia Antoniadi; Hao Zhou; Hongxia Chen; Apostolis Angelis; Maria Halabalaki; Leandros A Skaltsounis; Zhiwen Qi; Chengzhang Wang

doi:10.1016/j.foodchem.2024.142580

Sodium cholate-coated Olea europaea polyphenol nanoliposomes: Preparation, stability, release, and bioactivity

Food Chem. 2024 Dec 19:469:142580. doi: 10.1016/j.foodchem.2024.142580. Online ahead of print.

Authors

Wenjun Li¹, Lemonia Antoniadi², Hao Zhou¹, Hongxia Chen¹, Apostolis Angelis³, Maria Halabalaki³, Leandros A Skaltsounis³, Zhiwen Qi⁴, Chengzhang Wang⁵

Affiliations

¹ Institute of Chemical Industry of Forest Products, CAF, National Engineering Laboratory for Biomass Chemical Utilization, Key and Open Laboratory on Forest Chemical Engineering, SFA, Key Laboratory of Biomass Energy and Material, Nanjing 210042, Jiangsu Province, China.
² Division of Pharmacognosy and Natural Products Chemistry, National and Kapodistrian University of Athens, Panepistimioupoli Zografou, NKUA, 15771, Athens, Greece; Pharmagnose S.A., 57th km Athens-lamia National Road, Oinofyta 32011, Greece.
³ Division of Pharmacognosy and Natural Products Chemistry, National and Kapodistrian University of Athens, Panepistimioupoli Zografou, NKUA, 15771, Athens, Greece.
⁴ Institute of Chemical Industry of Forest Products, CAF, National Engineering Laboratory for Biomass Chemical Utilization, Key and Open Laboratory on Forest Chemical Engineering, SFA, Key Laboratory of Biomass Energy and Material, Nanjing 210042, Jiangsu Province, China. Electronic address: zhiwenqi89@163.com.
⁵ Institute of Chemical Industry of Forest Products, CAF, National Engineering Laboratory for Biomass Chemical Utilization, Key and Open Laboratory on Forest Chemical Engineering, SFA, Key Laboratory of Biomass Energy and Material, Nanjing 210042, Jiangsu Province, China. Electronic address: wangczlhs@sina.com.

PMID: 39721438
DOI: 10.1016/j.foodchem.2024.142580

Abstract

Ultra-flexible nanoliposomes (UNL) coated with sodium cholate were fabricated using the thin film hydration technique to encapsulate oleocanthal (OLEO), oleacein (OLEA), oleuropein (OLEU), and hydroxytyrosol (HT) for improving their stability and bioactivity. Their physicochemical properties were further validated through DLS, FTIR, XRD, TGA, and DSC analyses. Negative-staining TEM imaging revealed well-dispersed UNL with laminar vesicles inside. Additionally, their transdermal studies in vitro demonstrated that UNL enhanced the cumulative release of OLEO, OLEA, OLEU, and HT by 3.13, 2.76, 2.59, and 2.83 times, respectively. Furthermore, their release mechanisms were better approximated the Peppas-Sahlin model rather than the Korsmeyer-Peppas and Higuchi models, which governed by Fickian diffusion. Moreover, comparing to their compounds, UNL structure exhibited improved their antioxidant and cytotoxicity properties, highlighting their potential as effective delivery agents in humans. These results offer a novel approach for stabilizing biologically active polyphenols from Olea europaea, paving the way for enhanced human health applications.

Keywords: Olea europaea; Sodium cholate-coated; Transdermal properties, release mechanism; Ultra-flexible nanoliposomes.