An electrical characteristics extraction and analysis method for the membrane of medaka embryo during its development using electrical impedance spectroscopy

Lu Yang; Yang Wu; Songpei Hu; Jiafeng Yao; Fangming Chen

doi:10.1016/j.bioelechem.2024.108885

An electrical characteristics extraction and analysis method for the membrane of medaka embryo during its development using electrical impedance spectroscopy

Bioelectrochemistry. 2024 Dec 24:163:108885. doi: 10.1016/j.bioelechem.2024.108885. Online ahead of print.

Authors

Lu Yang¹, Yang Wu², Songpei Hu³, Jiafeng Yao⁴, Fangming Chen⁵

Affiliations

¹ School of Mechanical Engineering, Anhui University of Technology, Ma'anshan 243002, China. Electronic address: yanglu8823@163.com.
² College of Mechanical and Electrical Engineering, Nanjing Forestry University, Nanjing 210037, China. Electronic address: wuyang@njfu.edu.cn.
³ College of Mechanical & Electrical Engineering, Nanjing University of Aeronautics & Astronautics, Nanjing 210016, China. Electronic address: songpei.hu@nuaa.edu.cn.
⁴ College of Mechanical & Electrical Engineering, Nanjing University of Aeronautics & Astronautics, Nanjing 210016, China. Electronic address: jiaf.yao@nuaa.edu.cn.
⁵ Department of Biomedical Engineering, Fourth Military Medical University, Xi'an 710032, China. Electronic address: chenfangming@fmmu.edu.cn.

PMID: 39721369
DOI: 10.1016/j.bioelechem.2024.108885

Abstract

An electrical characteristics extraction and analysis method for membrane of medaka embryo during its development using Electrical Impedance Spectroscopy (EIS) is proposed. The proposed method is non-invasive, it doesn't affect the embryo's development. Embryo's equivalent electrical circuit (EEC) model is established to extract membrane's electrical characteristics, it is used to fit the embryo's electrical impedance curves in different developmental stages. The equivalent resistances and capacitances of membrane are regarded as the electrical characteristics to analyze the membrane's physiological changes during embryo's development. To achieve the embryo's electrical impedance curve from the measurement system exactly, an EIT-assisted electrical impedance curve extraction method is innovatively introduced. The extracted electrical characteristics of the membrane reflect its physiological changes well in both simulation and experiment. In experiment, the equivalent capacitances of embryo's membrane are increasing, while the equivalent resistances show a downward trend from neurula stage to hatching stage. The experiment results indicate that the permeability of membrane becomes higher and the thickness of membrane becomes thinner from neurula stage to hatching stage.

Keywords: Electrical Impedance Spectroscopy (EIS); Electrical Impedance Tomography (EIT); Electrical characteristics; Embryo membrane; Equivalent electrical circuit (EEC); Medaka embryo development.