A novel mouse model for studies of burn wound conversion using a top hat-shaped brass template

Norifumi Matsuda; Chigusa Sato; Kosuke Ishikawa; Takahiro Miura; Emi Funayama; Yuhei Yamamoto; Kanako Fuyama; Shunsuke Ohnishi; Taku Maeda

doi:10.1016/j.burns.2024.107348

A novel mouse model for studies of burn wound conversion using a top hat-shaped brass template

Burns. 2024 Dec 10;51(2):107348. doi: 10.1016/j.burns.2024.107348. Online ahead of print.

Authors

Norifumi Matsuda¹, Chigusa Sato², Kosuke Ishikawa¹, Takahiro Miura¹, Emi Funayama¹, Yuhei Yamamoto¹, Kanako Fuyama³, Shunsuke Ohnishi⁴, Taku Maeda⁵

Affiliations

¹ Department of Plastic and Reconstructive Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Kita 15, Nishi 7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan.
² Department of Plastic and Reconstructive Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Kita 15, Nishi 7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan; Department of Plastic and Reconstructive Surgery, Caress Sapporo Tokeidai Memorial Hospital, Kita 1, Higashi 1, Chuo-ku, Sapporo, Hokkaido 060-0031, Japan.
³ Data Science Center, Promotion Unit, Institute of Health Science Innovation for Medical Care, Hokkaido University Hospital, Kita 14, Nishi 5, Kita-ku, Sapporo, Hokkaido 060-8648, Japan.
⁴ Laboratory of Molecular and Cellular Medicine, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita-ku, Sapporo, Hokkaido 060-0812, Japan.
⁵ Department of Plastic and Reconstructive Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Kita 15, Nishi 7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan. Electronic address: takumaeda1105@yellow.plala.or.jp.

PMID: 39721234
DOI: 10.1016/j.burns.2024.107348

Abstract

Introduction: The pathophysiology of burn wound conversion is not fully understood. Animal models are needed to elucidate the underlying mechanisms and develop treatments. Here, we established a new reproducible mouse model that simulates this process, thereby facilitating studies of burn wound conversion.

Materials and methods: After anesthesia and depilation, 75 mice were randomly assigned to 5-, 15-, and 25-s contact groups, and a top hat-shaped brass template was heated in boiling water and applied to the lateral abdomen. The wound area was calculated from photographs and the percentage of the surviving area was determined. Histological samples were collected 1-96 h after injury.

Results: A 15-s contact time produced the desired 50 %-75 % interspace necrosis at 96 h after injury. The 5-s contact group had a mostly preserved interspace, while the 25-s contact group exhibited near-complete necrosis. Histologically, significant differences between the 5- and 15-s contact groups were seen in cutaneous appendage denaturation and panniculus carnosus denaturation early after injury.

Conclusion: Exposing mice to the heated template for 15 s provides a reproducible model for studying burn wound conversion mechanisms and treatments, facilitating further elucidation of burn pathophysiology and evaluation of therapies to prevent burn wound conversion.

Keywords: Burn wound conversion; Burn wound progressive deepening; Burns; Experimental, animal model; Laboratory animal models; Mice; Thermal injury.