Ultrasound-assisted extraction of polysaccharide from Allium chinense G. Don epidermal waste: Evaluation of extraction mechanism, physicochemical properties, and bioactivities

Jiangfei Li; Guanjun Ye; Zhilong Zhou; Jianlong Wang; Jiayu Wang; Ting Ju; Alejandro Cifuentes; Elena Ibañez; Denis Baranenko; Haitian Zhao; Weihong Lu

doi:10.1016/j.ultsonch.2024.107210

Ultrasound-assisted extraction of polysaccharide from Allium chinense G. Don epidermal waste: Evaluation of extraction mechanism, physicochemical properties, and bioactivities

Ultrason Sonochem. 2024 Dec 21:112:107210. doi: 10.1016/j.ultsonch.2024.107210. Online ahead of print.

Authors

Affiliations

¹ Department of Food Nutrition and Health, School of Medicine and Health, Faculty of Life Sciences and Medicine, Harbin Institute of Technology, Harbin 150001, PR China; Zhengzhou Research Institute, Harbin Institute of Technology, Zhengzhou 450003, PR China; National and Local Joint Engineering Laboratory for Synthesis, Transformation and Separation of Extreme Environmental Nutrients, Harbin Institute of Technology, Harbin 150001, PR China.
² Department of Food Nutrition and Health, School of Medicine and Health, Faculty of Life Sciences and Medicine, Harbin Institute of Technology, Harbin 150001, PR China; National and Local Joint Engineering Laboratory for Synthesis, Transformation and Separation of Extreme Environmental Nutrients, Harbin Institute of Technology, Harbin 150001, PR China.
³ Laboratory of Foodomics, Institute of Food Science Research, CIAL, CSIC, Nicolás Cabrera 9, Madrid 28049, Spain.
⁴ School of Life Sciences, International Research Centre Biotechnologies of the Third Millennium, ITMO University, St. Petersburg. 197101, Russia.
⁵ Department of Food Nutrition and Health, School of Medicine and Health, Faculty of Life Sciences and Medicine, Harbin Institute of Technology, Harbin 150001, PR China; National and Local Joint Engineering Laboratory for Synthesis, Transformation and Separation of Extreme Environmental Nutrients, Harbin Institute of Technology, Harbin 150001, PR China. Electronic address: zhaohaitian@hit.edu.cn.
⁶ Department of Food Nutrition and Health, School of Medicine and Health, Faculty of Life Sciences and Medicine, Harbin Institute of Technology, Harbin 150001, PR China; Zhengzhou Research Institute, Harbin Institute of Technology, Zhengzhou 450003, PR China; National and Local Joint Engineering Laboratory for Synthesis, Transformation and Separation of Extreme Environmental Nutrients, Harbin Institute of Technology, Harbin 150001, PR China. Electronic address: lwh@hit.edu.cn.

PMID: 39721204
DOI: 10.1016/j.ultsonch.2024.107210

Abstract

Allium chinense G. Don waste (ACGD) has an abundance of polysaccharides (ACGDP). Therefore, in this study, a method for extraction of ACGDP from ACGD by ultrasonic-assisted hot water extraction (UAE) based on ultrasonic cleaning equipment was developed. It was compared with hot water extraction (HWE) in terms of efficiency, energy consumption, and carbon emissions. Then, the mechanism of efficient extraction of ACGDP by UAE was further investigated. Finally, the physicochemical properties and in vitro activity of ACGDP were evaluated. The results suggested that the optimal conditions of UAE were temperature 53 ℃, time 34 min, power 280 W, liquid-solid ratio 22 mL/g, and the yield of ACGDP was 30.08 ± 0.21 %. Compared with HWE, UAE yield was increased by 30.10 %, time was shortened by 71.50 %, energy consumption was reduced by 77.33 %, and CO₂ emission was reduced by 82.56 %. UAE can efficiently extract ACGDP by changing the composition of the cell wall, destroying the microstructure, and reducing the particle size. In addition, ACGDP had better thermal stability and antioxidant activity, and exhibited positive hypoglycemic activity. Overall, this study provided a theoretical basis and new insights into the efficient extraction mechanism of UAE and the high-value utilization of ACGD waste.

Keywords: Biomass waste; Circular economy; Extraction mechanism; Polysaccharide; Ultrasound-assisted extraction.