Rapid on-site diagnosis of PEDV and PoRV co-infection by gold magnetic nanoparticles-based SERS immunochromatography

Zhaoyuan He; Xiaoge Duan; Zhi Zhao; Yingkai Chen; Chengxiang Fu; Fuxin Zhang; Jinzi Wang; Jianyuan Feng; Nanxin Lin; Hailan Chen

doi:10.1016/j.talanta.2024.127428

Rapid on-site diagnosis of PEDV and PoRV co-infection by gold magnetic nanoparticles-based SERS immunochromatography

Talanta. 2024 Dec 19:285:127428. doi: 10.1016/j.talanta.2024.127428. Online ahead of print.

Authors

Zhaoyuan He¹, Xiaoge Duan¹, Zhi Zhao¹, Yingkai Chen¹, Chengxiang Fu¹, Fuxin Zhang¹, Jinzi Wang², Jianyuan Feng³, Nanxin Lin⁴, Hailan Chen⁵

Affiliations

¹ College of Animal Science and Technology, Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Guangxi University, Nanning, 530004, China.
² School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning, 530006, Guangxi, China.
³ Guangxi State Farms Yongxin Animal Husbandry Group Co., Ltd., Nanning, 530032, Guangxi, China.
⁴ School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning, 530006, Guangxi, China. Electronic address: NanxinLinGXMZU@163.com.
⁵ College of Animal Science and Technology, Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Guangxi University, Nanning, 530004, China. Electronic address: hlchen319@163.com.

PMID: 39719732
DOI: 10.1016/j.talanta.2024.127428

Abstract

Porcine epidemic diarrhea virus (PEDV) and porcine rotavirus (PoRV) are the two main pathogens causing porcine diarrhea, which are characterized by high morbidity and mortality. Most of the diagnostic methods available are limited to the laboratory or fail to highlight their advantages in terms of target species, detection time, sensitivity, and stability. To meet the demand for rapid on-site diagnosis of PEDV and PoRV co-infection, a surface-enhanced Raman scattering (SERS) immunochromatographic sensor based on gold magnetic nanoparticles (MNPs) was developed. The sensor is dual-mode, detecting on the basis of color signals by the naked eye and Raman signals. After a series of optimizations, the constructed sensor could perform simultaneous qualitative and quantitative detection of PEDV and PoRV in just 18 min, with visualized (color signals observed by the naked eye) limits of detection (LOD) of 3.13 × 10² TCID₅₀/mL and 4.69 × 10² copies/μL, respectively. The LOD based on Raman signal analysis was as low as 4.63 × 10¹ TCID₅₀/mL and 3.30 × 10² copies/μL for PEDV and PoRV, respectively. In addition, the sensor exhibited excellent specificity without cross-reactivity with common pathogens. The overall compliance rate with RT-PCR was 92.1 % (35/38) for 38 clinical samples. Therefore, the sensor is characterized by high sensitivity, specificity, reproducibility, and accuracy, making it suitable for the simultaneous rapid on-site detection of PEDV and PoRV.

Keywords: Gold magnetic nanoparticles; Porcine epidemic diarrhea virus; Porcine rotavirus; SERS immunochromatography; Simultaneous diagnosis.