A Gram-stain-negative, aerobic, non-spore-forming, non-motile, coccus-shaped, and red-pigmented bacterial strain designated as CJ14T was isolated from lettuce cultivation soil in Yong-In, South Korea. Strain CJ14T grew optimally on Luria-Bertani agar at 37 ℃ and pH 7.0 in the absence of NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ14T belonged to the genus Rhodoligotrophos in the family Rhodoligotrophaceae and was closely related to Rhodoligotrophos defluvii lm1T (98.4% similarity). The genome size of strain CJ14T was 4.8 Mbp consisting of a single contig, and the DNA G + C content of strain CJ14T was 61.8%. The genomic comparison of strain CJ14T to R. defluvii lm1T showed an average nucleotide identity value of 77.9%, and the digital DNA-DNA hybridization value was 22.4%. Genomic analyses revealed that strain CJ14T possessed a gene cluster for ochrobactin biosynthesis, highly homologous to the siderophore gene cluster from Nitratireductor thuwali Nit1536T. Strain CJ14T contained ubiquinone (Q-10) as the predominant respiratory quinone. The major cellular fatty acids of strain CJ14T were C16:0, C19:0 cyclo ω8c and summed feature 8. The polar lipid profile was composed of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, one unidentified aminolipid, one unidentified glycolipid and five unidentified lipids. Based on polyphasic taxonomy study, strain CJ14T could be classified as a novel species of the genus Rhodoligotrophos, for which the name Rhodoligotrophos ferricapiens sp. nov. is proposed. The type strain is CJ14T (= KACC 23063T = JCM 36057T).
Keywords: Rhodoligotrophos ferricapiens; 16S rRNA sequences; Bacterial genome; Novel species; Siderophore.
© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.