Proposal of one new family, seven new genera and seventy new basidiomycetous yeast species mostly isolated from Tibet and Yunnan provinces, China

Abstract

More than 2 000 yeast strains isolated from 1 200 samples mostly collected from Tibet and Yunnan provinces in China were identified as 462 species according to the internal transcribed spacer including the 5.8S rDNA (ITS) and the D1/D2 domains of the large subunit rDNA (LSU) sequence analyses. Among them, 70 new basidiomycetous yeast species were proposed based on the multi-locus phylogenetic analyses including the D1/D2 domains, the ITS, the small subunit rDNA (SSU), the largest subunit of RNA polymerase II (RPB1), the second largest subunit of RNA polymerase II (RPB2) and translation elongation factor 1-α (TEF1), as well as the phenotypic comparisons. The average nucleotide identity (ANI) analysis with the genomic metric was also used in the evaluation of the species delimitation for the genera Holtermannia, Mrakia and Takashimella that contain closely related species with low sequence heterogeneity in both ITS and D1/D2 regions. Forty-six new species belonged to 16 genera in the Agaricomycotina, 13 new species occurred in 12 genera in the Pucciniomycotina, three new species were distributed in three genera in the Ustilaginomycotina, and eight new species were classified in seven newly established genera. One new family was also proposed based on one of these new genera. The analyses revealed several inaccurate species names attributed to genomes deposited in GenBank, indicating the necessity of a more rigorous quality checks of the genomes deposited in the public databases. Taxonomic novelties: New family: Vankyiozymaceae Q.-M. Wang; New genera: Baiomyces Q.-M. Wang, Bauerozyma Q.-M. Wang, Fengyania Q.-M. Wang, Foliozyma Q.-M. Wang, Litoriozyma Q.-M. Wang, Nakaseozyma Q.-M. Wang, Vankyiozyma Q.-M. Wang; New species: Baiomyces sejilaensis Q.-M. Wang, Bauerozyma artemisiae Q.-M. Wang, Boekhoutia foliicola Q.-M. Wang, Buckleyzyma pseudoaurantiaca Q.-M. Wang, Carlosrosaea betulae Q.-M. Wang, Carlosrosaea rhododendri Q.-M. Wang, Carlosrosaea yunnanensis Q.-M. Wang, Chrysozyma quercicola Q.-M. Wang, Curvibasidium pini Q.-M. Wang, Cystobasidium cunninghamiae Q.-M. Wang, Derxomyces foliicola Q.-M. Wang, Derxomyces motuoensis Q.-M. Wang, Derxomyces orientalis Q.-M. Wang, Derxomyces paracylindricus Q.-M. Wang, Exobasidium lijiangense Q.-M. Wang, Fengyania pteridophytorum Q.-M. Wang, Foliozyma liliicola Q.-M. Wang, Halobasidium phyllophilum Q.-M. Wang, Hannaella artemisiae Q.-M. Wang, Hannaella pteridophytorum Q.-M. Wang, Hannaella urticae Q.-M. Wang, Holtermannia pseudosaccardoi Q.-M. Wang, Kockovaella cariosiligni Q.-M. Wang, Kockovaella foliicola Q.-M. Wang, Kondoa tibetensis Q.-M. Wang, Kwoniella hippophaes Q.-M. Wang, Kwoniella lonicerae Q.-M. Wang, Litoriozyma hainanensis Q.-M. Wang, Meira marina Q.-M. Wang, Microsporomyces betulae Q.-M. Wang, Microsporomyces foliicola Q.-M. Wang, Mrakia pini Q.-M. Wang, Mrakia rhododendri Q.-M. Wang, Nakaseozyma junci Q.-M.Wang, Nakaseozyma lonicerae Q.-M. Wang, Papiliotrema castaneae Q.-M. Wang, Papiliotrema catalpae Q.-M. Wang, Phaeotremella pini Q.-M. Wang, Phaffia paratasmanica Q.-M. Wang, Phaffia rhododendri Q.-M. Wang, Piskurozyma cuscutae Q.-M. Wang, Piskurozyma humicola Q.-M. Wang, Piskurozyma liliaceifoliae Q.-M. Wang, Piskurozyma linzhiensis Q.-M. Wang, Piskurozyma nanyiensis Q.-M. Wang, Piskurozyma terricola Q.-M. Wang, Pseudohyphozyma sanghuangpori Q.-M. Wang, Pseudotremella hippophaes Q.-M. Wang, Pseudotremella rhododendri Q.-M. Wang, Rhodotorula linzhiensis Q.-M. Wang, Slooffia terricola Q.-M. Wang, Takashimella corticis Q.-M. Wang, Teunia betulicola Q.-M. Wang, Teunia chimonanthi Q.-M. Wang, Teunia heritierae Q.-M. Wang, Teunia myricariae Q.-M. Wang, Teunia parabetulicola Q.-M. Wang, Teunia quercus Q.-M. Wang, Teunia rhododendri Q.-M. Wang, Ustilago foliicola Q.-M. Wang, Vankyiozyma motuoensis Q.-M. Wang, Vanrija silvicola Q.-M. Wang, Vishniacozyma catalpae Q.-M. Wang, Vishniacozyma marinae Q.-M. Wang, Vishniacozyma paravictoriae Q.-M. Wang, Vishniacozyma pini Q.-M. Wang, Vishniacozyma pyri Q.-M. Wang, Vishniacozyma sinopodophylli Q.-M. Wang, Vishniacozyma zhenxiongensis Q.-M. Wang, Yurkovia castaneae Q.-M. Wang. Citation: Jiang Y-L, Bao W-J, Liu F, Wang G-S, Yurkov AM, Ma Q, Hu Z-D, Chen X-H, Zhao W-N, Li A-H, Wang Q-M (2024). Proposal of one new family, seven new genera and seventy new basidiomycetous yeast species mostly isolated from Tibet and Yunnan provinces, China. Studies in Mycology 109: 57-153. doi: 10.3114/sim.2024.109.02.

Keywords: Average nucleotide identity (ANI); basidiomycetous yeasts; genomic metrics; molecular phylogeny; seventy-eight new taxa.