The association between dietary intake of branched-chain amino acids and the odds of nonalcoholic fatty liver disease among overweight and obese children and adolescents

Ali Nikparast; Maryam Razavi; Mohammad Hassan Sohouli; Azita Hekmatdoost; Pooneh Dehghan; Maryam Tohidi; Pejman Rouhani; Golaleh Asghari

doi:10.1007/s40200-024-01516-1

The association between dietary intake of branched-chain amino acids and the odds of nonalcoholic fatty liver disease among overweight and obese children and adolescents

J Diabetes Metab Disord. 2024 Dec 20;24(1):19. doi: 10.1007/s40200-024-01516-1. eCollection 2025 Jun.

Authors

Ali Nikparast^{1

2}, Maryam Razavi³, Mohammad Hassan Sohouli², Azita Hekmatdoost¹, Pooneh Dehghan⁴, Maryam Tohidi⁵, Pejman Rouhani², Golaleh Asghari¹

Affiliations

¹ Department of Clinical Nutrition and Dietetics, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
² Pediatric Gastroenterology and Hepatology Research Center Pediatrics Centre of Excellence Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
³ Growth and development research center, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
⁴ Department of Imaging, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
⁵ Prevention of Metabolic Disorders Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

PMID: 39712343
PMCID: PMC11659539 (available on 2025-12-20)
DOI: 10.1007/s40200-024-01516-1

Abstract

Objectives: Dietary supplementation with branched-chain amino acids (BCAAs), including leucine, isoleucine, and valine, has shown potential benefits for the metabolic profile. However, emerging population-based studies suggest that BCAAs may mediate pathways related to cardiometabolic risk factors, possibly due to their involvement in the dysregulation of insulin metabolic pathways. This study aimed to investigate the association between BCAAs intake and the odds of nonalcoholic fatty liver disease (NAFLD) in children and adolescents with overweight and obesity.

Methods: This cross-sectional study encompassed individuals aged 6 to 18 years with WHO body mass index (BMI)-for-age z-score ≥ 1. NAFLD diagnosis was done using an ultrasonography scan of the liver and gastroenterologist confirmation. Dietary BCAAs intake was assessed using a validated 147-item food frequency questionnaire. Logistic regression models, adjusted for potential confounders, were used to estimate the odds ratios (OR) and 95% confidence interval (CI) of NAFLD across quartiles of BCAAs intake.

Results: A total of 505 (52.9% boys) with mean ± SD age and BMI-for-age-Z-score of 10.0 ± 2.3 and 2.70 ± 1.01, respectively, were enrolled. After adjusting for potential confounders, participants in the highest quartile of total dietary BCAAs (OR: 1.87;95%CI:1.06-3.28) and leucine (OR: 1.84;95%CI:1.03-3.29) intake had greater odds of developing NAFLD compared with those in the lowest quartile. There was no significant association between dietary valine and isoleucine intake and the odds of NAFLD.

Conclusions: The study findings suggest that increased dietary intake of BCAAs, particularly leucine, may have detrimental effects on the development of NAFLD.

Keywords: Branched-chain amino acid intake; Children; Insulin resistance; Nonalcoholic fatty liver disease; Overweight and obesity.

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