Photosynthetic traits, water use and the yield of maize are influenced by soil water stability

Abstract

Background: The aim of this study is to investigate the effect of soil water stability on maize (Zea mays L.) yield, water use, and its photosynthetic physiological mechanisms, and to innovate the relationship between maize and soil water, which currently only considers soil water content and neglects soil water stability.

Methods: An organized water experiment was conducted on maize. The effects of stable soil water (SW) at two water content levels were examined, with fluctuating soil water (FW) as a control. The assessed effects included leaf water, chlorophyll, gas exchange, leaf water use efficiency (WUE), stable carbon isotope ratio (δ¹³C), and yield of maize.

Results: Soil water stability had a significant effect on maize yield, yet it was slightly smaller than soil water content. Compared with FW, SW increased the maximum net photosynthetic rate, saturated light intensity, stomatal conductance, SPAD, leaf water content, and leaf WUE, and decreased δ¹³C, promoting dry matter assimilation and conversion into grain yield, ultimately increasing yield by 100.8%. Under the same soil water stability, 55% FC versus 75% FC weakened photosynthetic capacity and exacerbated stomatal limitation of maize leaves, making them more susceptible to light inhibition, which decreased photoassimilate accumulation, resulting in a significant decrease in yield. And the δ¹³C under 75% FC conditions decreased by 4.7-7.7% compared with 55% FC.

Conclusion: In conclusion, SW exhibits a positive effect on maize leaf water content, photosynthetic carbon assimilation, and grain yields, regardless of soil water content. Compared to FW, SW increased leaf WUE and maize yield by enhancing photosynthesis, and SW has stronger discrimination against ¹³C during photosynthetic CO₂ assimilation, thus decreasing leaf δ¹³C. This study fills a gap in understanding how soil water stability influences maize yield and gas exchange, and provides a fresh perspective on how to improve crop yield and WUE by managing soil water stability.

Keywords: Light response curve; Maize; Photosynthetic traits; Soil water stability; Stable carbon isotopes; Water use efficiency.