Fabrication of hybrid nanocomposites for electrochemical evaluation of food-based preservative and bioactive targets of hydrogen peroxide and rutin in real fruit and drug samples

Chinnathambi Nandhini; Chi-Hsien Huang; Ponnusamy Arul; Sheng-Tung Huang

doi:10.1016/j.foodchem.2024.142502

Fabrication of hybrid nanocomposites for electrochemical evaluation of food-based preservative and bioactive targets of hydrogen peroxide and rutin in real fruit and drug samples

Food Chem. 2024 Dec 16:469:142502. doi: 10.1016/j.foodchem.2024.142502. Online ahead of print.

Authors

Chinnathambi Nandhini¹, Chi-Hsien Huang², Ponnusamy Arul³, Sheng-Tung Huang³

Affiliations

¹ Department of Materials Engineering, Ming Chi University of Technology, New Taipei City 24303, Taiwan.
² Department of Materials Engineering, Ming Chi University of Technology, New Taipei City 24303, Taiwan; Center for Plasma and Thin Film Technologies, Ming Chi University of Technology, New Taipei City 243303, Taiwan; College of Engineering, Chang Gung University, Taoyuan City 33302, Taiwan; Division of Rheumatology, Allergy and Immunology, Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan. Electronic address: chhuang@mail.mcut.edu.tw.
³ Institute of Biochemical and Biomedical Engineering, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, 10608, Taiwan.

PMID: 39708649
DOI: 10.1016/j.foodchem.2024.142502

Abstract

Development of a reliable tool to detect hydrogen peroxide (H₂O₂) and rutin in food-derived products and bioactive flavonoids is essential for food safety. Nevertheless, food/drug-based real samples are complex matrices that affect the sensor's specificity and sensitivity. For this purpose, we developed a simple electrochemical detection platform using covalent organic framework‑silver nanoparticles (COF-AgNPs). Based on spectral and electrochemical tests, COF-AgNPs displayed enhanced electroactive sites and facile electron transfer. For H₂O₂ and rutin, the designed sensor surface exhibited outstanding concentration linearity of 0.5 nM-1000 μM and 1 nM-900 μM, respectively, along with superior detection limits of 0.126 nM and 0.133 nM. Additionally, it demonstrated acceptable reproducibility and interference capability. In practical analysis, H₂O₂ and rutin were detected in milk, fruits, and drug samples with high recovery rates of 94.60-99.31 % (n = 3). Consequently, the designed sensor is ideal for screening targets for H₂O₂ and rutin in food sources and for food research.

Keywords: Bioactive flavonoid; Fruit samples; Hybrid composites; Hydrogen peroxide; Rutin.