The gastrointestinal absorption characteristics and metabolic mechanisms of polysaccharides from Hohenbuehelia serotina

Mian Wei; Huipeng Zhu; Qiang Zhang; Dawei Gao; Lu Wang; Xiaoyu Li

doi:10.1016/j.foodchem.2024.142483

The gastrointestinal absorption characteristics and metabolic mechanisms of polysaccharides from Hohenbuehelia serotina

Food Chem. 2024 Dec 15:468:142483. doi: 10.1016/j.foodchem.2024.142483. Online ahead of print.

Authors

Mian Wei¹, Huipeng Zhu¹, Qiang Zhang¹, Dawei Gao¹, Lu Wang², Xiaoyu Li³

Affiliations

¹ Nano-Biotechnology Key Laboratory of Hebei Province, State Key Laboratory of Metastable Materials Science and Technology, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, PR China.
² Nano-Biotechnology Key Laboratory of Hebei Province, State Key Laboratory of Metastable Materials Science and Technology, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, PR China. Electronic address: wl@ysu.edu.cn.
³ Nano-Biotechnology Key Laboratory of Hebei Province, State Key Laboratory of Metastable Materials Science and Technology, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, PR China. Electronic address: lixiaoyu@ysu.edu.cn.

PMID: 39706124
DOI: 10.1016/j.foodchem.2024.142483

Abstract

In order to elucidate the digestion, absorption and metabolism mechanism, Hohenbuehelia serotina polysaccharides were successfully labelled by fluorescein isothiocyanate (FITC-Tyr-HSP), with the fluorescent substitution degree of 0.37 %. FITC-Tyr-HSP with excellent physiological stability possessed the significant fluorescent characteristics, which could be dynamically monitored by in vivo fluorescence imaging and high performance gel permeation chromatography-fluorescence detector (HPGPC-FLD). Through investigation of gastrointestinal digestion in vitro, the degradation of HSP was only occurred in small intestinal fluid. The results of in vivo studies also confirmed that a small amount of HSP were degraded in small intestine, and then absorbed by intestinal epithelial cells to enter into the bloodstream, reaching to liver, spleen and kidney. Meanwhile, most of HSP that could not be digested were directly transported to cecum and colon, where they were metabolized by gut microbiota to produce SCFAs, with the total content of which reached to 60.52 mmol/L after gavage for 24 h. Ultimately, the polysaccharides were excreted from the body mainly by feces and urine pathways. This study is meaningful for providing a practical basis to elucidate the digestion, absorption and metabolism of polysaccharides.

Keywords: Absorption; Digestion; Fluorescent labeling; Hohenbuehelia serotina polysaccharides; Metabolism.