Transplantation of Islet Organoids into Brown Adipose Tissue in a Diabetic Mouse Model

Aixia Sun; Mankirat Singh; Manvir Bamrah; Wen Li; Aitor Aguirre; Ping Wang

doi:10.1007/7651_2024_588

Transplantation of Islet Organoids into Brown Adipose Tissue in a Diabetic Mouse Model

Methods Mol Biol. 2024 Dec 21. doi: 10.1007/7651_2024_588. Online ahead of print.

Authors

Aixia Sun^{1

2}, Mankirat Singh^{1

3}, Manvir Bamrah^{1

3}, Wen Li^{4

5}, Aitor Aguirre^{4

6}, Ping Wang^{7

8}

Affiliations

¹ Precision Health Program, Michigan State University, East Lansing, MI, USA.
² Department of Radiology, College of Human Medicine, Michigan State University, East Lansing, MI, USA.
³ Lyman Briggs College, Michigan State University, East Lansing, MI, USA.
⁴ Institute for Quantitative Health Science and Engineering (IQ), Michigan State University, East Lansing, MI, USA.
⁵ Department of Electrical and Computer Engineering, College of Engineering, Michigan State University, East Lansing, MI, USA.
⁶ Department of Biomedical Engineering, College of Engineering, Michigan State University, East Lansing, MI, USA.
⁷ Precision Health Program, Michigan State University, East Lansing, MI, USA. wangpin4@msu.edu.
⁸ Department of Radiology, College of Human Medicine, Michigan State University, East Lansing, MI, USA. wangpin4@msu.edu.

PMID: 39702860
DOI: 10.1007/7651_2024_588

Abstract

Pancreatic islet transplantation is a promising cell replacement therapy for patients with type 1 diabetes (T1D), an autoimmune disease that destroys insulin-producing islet β cells. However, the shortage of donor pancreatic islets significantly limits the widespread use of this strategy as a routine therapy. Pluripotent stem cell-derived insulin-producing islet organoids present a promising alternative β cell source for T1D patients. One critical challenge is the lack of vascularization in islet organoids, making it essential to investigate vascularized transplantation sites to support their survival. Brown adipose tissue (BAT) is well vascularized and secretes active cytokines, facilitating islet organoid survival. Thus, BAT represents a promising transplantation site for islet organoids, making it an ideal location to support cell replacement therapies and improve treatment approaches for T1D. Here, we describe the methods for transplanting human-induced pluripotent stem cell (iPSC)-derived islet organoids into the BAT of a mouse model.

Keywords: Brown adipose tissue (BAT); Islet organoids; Islet organoids transplantation; Pluripotent stem cell; Stem cell differentiation; Type 1 diabetes; β cells.