The in ovo screening of 27 single essential oils showed selective effects on hatchability, performance and gene expression relevant to gut functions in broilers at hatch

Asad A Khaskheli; Shahram Niknafs; Mila M Y Meijer; Xinle Tan; Peter R Ferket; Eugeni Roura

doi:10.1016/j.psj.2024.104670

The in ovo screening of 27 single essential oils showed selective effects on hatchability, performance and gene expression relevant to gut functions in broilers at hatch

Poult Sci. 2024 Dec 12;104(1):104670. doi: 10.1016/j.psj.2024.104670. Online ahead of print.

Authors

Asad A Khaskheli¹, Shahram Niknafs¹, Mila M Y Meijer¹, Xinle Tan¹, Peter R Ferket², Eugeni Roura³

Affiliations

¹ Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Australia.
² Prestage Department of Poultry Science, North Carolina State University, Raleigh, USA.
³ Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Australia. Electronic address: e.roura@uq.edu.au.

PMID: 39693964
DOI: 10.1016/j.psj.2024.104670

Abstract

The early post-hatching phase remains to be one of the most vulnerable phases in broiler production. Some essential oils have been reported to improve gut health and growth in broiler chickens when applied to post-hatching diets. However, in-feed applications are unable to prevent the health challenges observed immediately after hatching. Thus, pre-hatch interventions need to be considered. A research project was developed with the aim of investigating the impact of in ovo application of 27 selected essential oils (EOs) on foetal development with emphasis on gut integrity in broiler hatchlings. The eggs were incubated under standard conditions until day 17.5, when 1 mL of each EO preparation (5 µL EO + 5 µL polysorbate-80 + 990 µL saline) was injected into the amnion. Hatchability, body weight and organ weights (residual yolk, gizzard-proventriculus, intestines, liver, and heart) were measured at hatch. Five essential oils eugenol, clove, tea tree, lemongrass, and thyme, significantly (P < 0.05) reduced hatchability (66.67 %, 58.33, 83.30 and 83.30 %) compared to the saline (96.80 %), were discarded from the rest of the study. The other 22 essential oils were investigated in a second phase to assess their impact on expression of gut biomarkers including: a) jejunum integrity; b) digestive enzymes and nutrient transporters; and c) immune system. The results indicated that lemon myrtle significantly increased and oregano EO decreased body weight at hatch (BW0) compared to the saline (P < 0.05). Ylang ylang, clary sage, bergamot, lemon myrtle, and black pepper upregulated the expressions of biomarkers regulating gut integrity and barrier functions (ZO-1, ZO-2, CLDN1, MARVELD2, EGFR and EGF), nutrients transporters (EAAT3, PEPT1, I-FABP1, SGLT1), and digestive enzymes (APN, SI). Ylang ylang, turmeric acid, star anise, clary sage, and black pepper upregulated the expression of gut immunity biomarkers IL1B, IL10, IGMH, CD3D, and BU1 compared to the saline. In conclusion, in ovo delivery of selected EOs has the potential to improve embryonic development relevant to nutrient digestion and absorption, gut integrity and immunity in broilers.

Keywords: Digestive enzymes; Essential oils; Gut integrity; Immune system; in ovo.