Electrochemiluminescence immunosensor using a lanthanum-based metal-organic framework as signal probe and Cu2MoS4 as a co-reaction promoter for the sensitive detection of anti-Müllerian hormone

Jing Li; Shenglan Hu; Lingling Zheng; Lixin Xu; Yusheng Wu; Biyang Deng

doi:10.1016/j.talanta.2024.127406

Electrochemiluminescence immunosensor using a lanthanum-based metal-organic framework as signal probe and Cu₂MoS₄ as a co-reaction promoter for the sensitive detection of anti-Müllerian hormone

Talanta. 2024 Dec 16:285:127406. doi: 10.1016/j.talanta.2024.127406. Online ahead of print.

Authors

Jing Li¹, Shenglan Hu¹, Lingling Zheng¹, Lixin Xu¹, Yusheng Wu¹, Biyang Deng²

Affiliations

¹ State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), Collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, 541004, China.
² State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), Collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, 541004, China. Electronic address: dengby16@163.com.

PMID: 39693866
DOI: 10.1016/j.talanta.2024.127406

Abstract

In this study, we used meso-tetra (4-carboxyphenyl) porphyrin (TCPP) as an organic ligand to modify a lanthanide-based metal-organic framework as an electrochemiluminescence (ECL) platform to sensitively detect anti-Müllerian hormone (AMH). La-MOF amplified the ECL signal by suppressing the aggregation-caused quenching created by TCPP self-aggregation. Utilizing the reversible cycling of the mixed-valence transition metal ion (Cu⁺/Cu²⁺ and Mo⁴⁺/Mo⁶⁺) and the electrical conductivity of Cu₂MoS₄ and silver nanoparticle (AgNP), Cu₂MoS₄-AgNP as a dual co-reaction promoter constantly generated sulfate radical anions (SO₄^•-) and thus amplified the ECL signal. Based on this strategy, we built a sandwich-type ECL immunosensor for the sensitive detection of AMH with a linear range of 1 × 10^-4 to 50 ng/mL and a limit of detection of 24 fg/mL. This study provides a novel approach for the sensitive and selective detection of AMH, demonstrating its high potential and practical value for the sensitive detection of AMH.

Keywords: Aggregation-caused quenching; Co-reaction promoter; Electrochemiluminescence sensor; Lanthanide-based metal-organic framework; anti-Müllerian hormone.