1,3-Dithiolane as a Privileged Scaffold in Bioactive Derivatives: Chiral Resolution and Assignment of Absolute Configuration

Int J Mol Sci. 2024 Nov 29;25(23):12880. doi: 10.3390/ijms252312880.

Abstract

The 1,3-dithiolane ring has been recently rehabilitated as a chemical scaffold in drug design. However, for derivatives that are substituted in position 4, the introduction of a chiral center on the heterocycle demands the separation and characterization of the stereoisomers. We report the first chiral resolution and absolute configuration (AC) assignment for (1,4-dithiaspiro[4.5]decan-2-yl)methanol (R/S)-1, a key synthon for dithiolane-based biologically active compounds. Using (semi)preparative enantioselective HPLC, we isolated enantiomeric 1. The AC was assigned by using (+)-1 for the enantioselective synthesis of (+)-BS148, a sigma receptor modulator. An X-ray diffraction analysis established the (R)-configuration of (+)-BS148 and, by extension, of (+)-1. This method provides a reliable approach for preparing enantiopure 1,3-dithiolane scaffolds and establishes reference standards for AC determination of related compounds.

Keywords: 1,3-dithiolane; X-ray diffraction; absolute configuration assignment; chiral resolution; enantioselective chromatography.

MeSH terms

  • Chromatography, High Pressure Liquid / methods
  • Crystallography, X-Ray / methods
  • Molecular Structure
  • Receptors, sigma* / metabolism
  • Stereoisomerism

Substances

  • Receptors, sigma

Grants and funding

This work was supported by MUR (Ministero dell’Università e della Ricerca) and PON R&I 2014-2020-Asse IV “Istruzione e Ricerca per il recupero-REACT-EU,” Azione IV.6 “Contratti di Ricerca su tematiche Green”.