A turn-on fluorescent probe for imaging of hydroxyl radicals in drug-induced liver injury

Mengshan Yu; Hanyue Xiang; Ayijiang Taledaohan; Wenzhuo Fu; Hongyu Li; Yuji Wang; Nao Xiao

doi:10.1016/j.saa.2024.125569

A turn-on fluorescent probe for imaging of hydroxyl radicals in drug-induced liver injury

Spectrochim Acta A Mol Biomol Spectrosc. 2024 Dec 9:329:125569. doi: 10.1016/j.saa.2024.125569. Online ahead of print.

Authors

Mengshan Yu¹, Hanyue Xiang¹, Ayijiang Taledaohan¹, Wenzhuo Fu², Hongyu Li³, Yuji Wang¹, Nao Xiao⁴

Affiliations

¹ Beijing Area Major Laboratory of Peptide and Small Molecular Drugs, Engineering Research Center of Endogenous Prophylactic of Ministry of Education of China, School of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, China.
² Core Facilities Center, Capital Medical University, Beijing 100069, China.
³ College of Pharmacy, Zunyi Medical University, Zunyi 563003, China. Electronic address: lihongyu@iccas.ac.cn.
⁴ Beijing Area Major Laboratory of Peptide and Small Molecular Drugs, Engineering Research Center of Endogenous Prophylactic of Ministry of Education of China, School of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, China. Electronic address: xiaonao@ccmu.edu.cn.

PMID: 39671817
DOI: 10.1016/j.saa.2024.125569

Abstract

Hydroxyl radical is the most oxidizing type of reactive oxygen species (ROS), which is closely related to body health. In this work, a novel turn-on fluorescent probe BIJ-H for OH with high sensitivity was reported. BIJ-H was synthesized by a three-step reaction, and the pure product was obtained by only washing with poor solvents. In a neat aqueous solution containing BIJ-H, after adding OH, the probe was oxidized to BIJ, which produced fluorescence emission at 625 nm under the excitation with 550 nm. This sensing behavior can be achieved under physiological pH conditions. The detection limit of BIJ-H for OH was as low as 0.1379 μM. Thanks to the good analytical properties of BIJ-H, the probe has been applied to fluorescence imaging of OH in HepG2 cells and in acute liver injury induced by acetaminophen. The probe BIJ-H is expected to be used in the qualitative and quantitative detection of OH in physiological or pathological processes.

Keywords: APAP-induced liver injury; Cell imaging; Fluorescence probe; Hydroxyl radicals; Tissue fluorescence imaging.