Here, we report the genome sequence of Burkholderia ambifaria BCC0191, a biopesticide originally isolated from the barley rhizosphere. The genome was assembled using an Illumina-Nanopore hybrid approach and consisted of 7.62 Mbp distributed across three replicons. Several specialized metabolite biosynthetic gene clusters, including those known to be active in biocontrol, were identified.
Keywords: Burkholderia; biocontrol; cepacin; genome mining.