Validation of the 11+ Myco MS-PREP® Method for Determination of Aflatoxins, Fumonisins, Deoxynivalenol, Ochratoxin A, Zearalenone, HT-2, and T-2 Toxins in Cereals, Baby Food, Spices, and Animal Feed by Immunoaffinity Column with LC-MS/MS: AOAC Performance Tested MethodsSM 112401

Dave Leeman; Andrew B Allan; Helen Cameron; Carol Donelly; Adam Tramaseur; Joanna Stratton; Susan J MacDonald

doi:10.1093/jaoacint/qsae097

Validation of the 11+ Myco MS-PREP® Method for Determination of Aflatoxins, Fumonisins, Deoxynivalenol, Ochratoxin A, Zearalenone, HT-2, and T-2 Toxins in Cereals, Baby Food, Spices, and Animal Feed by Immunoaffinity Column with LC-MS/MS: AOAC Performance Tested MethodsSM 112401

J AOAC Int. 2024 Dec 11:qsae097. doi: 10.1093/jaoacint/qsae097. Online ahead of print.

Authors

Dave Leeman¹, Andrew B Allan¹, Helen Cameron¹, Carol Donelly¹, Adam Tramaseur², Joanna Stratton², Susan J MacDonald²

Affiliations

¹ R-Biopharm Rhône Ltd Block 10 Todd Campus, West of Scotland Science Park, Acre Road, Glasgow, Scotland G20 0XA.
² Fera Science Ltd Sand Hutton, York, YO41 1LZ.

PMID: 39661480
DOI: 10.1093/jaoacint/qsae097

Abstract

Background: The 11+Myco MS-PREP® Immunoaffinity Column (IAC) contains a gel suspension of monoclonal antibodies specific to the toxins of interest. Following sample extraction, the IAC is used for cleanup and concentration of mycotoxins prior to analysis by Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS).

Objective: This study evaluated the IAC with LC-MS/MS method for Performance Tested Methods SM certification for simultaneous determination and confirmation of Aflatoxins B1, B2, G1, G2, and M1; Deoxynivalenol, Fumonisins B1, B2, and B3; Ochratoxin A; T-2; HT-2; and Zearalenone from corn, wheat, cereal-based baby food (with and without dairy ingredients), paprika, chili powder and animal feed.

Methods: A single extraction method using 50% Acetonitrile and water was used for all matrixes. The Method Developer validated all matrixes and an independent laboratory verified method performance on corn and animal feed. Data were analyzed for recovery, repeatability precision, LOD, LOQ, confirmation of identity, and method selectivity.

Results: Recovery (72%-138%) and repeatability (0.46%-24%), with the exception of sporadic data points, were within acceptance criteria. LOQ was estimated as AFB1 0.018-0.32 ꙡg/kg, AFB2 0.037-0.28 ꙡg/kg, AFG1 0.019-0.14 ꙡg/kg, AFG2 0.036-0.28 ꙡg/kg, DON 4.0-75 ꙡg/kg, Fumonisin B1 4.9-37 ꙡg/kg, Fumonisin B2 4.0-32 ꙡg/kg, Fumonisin B3 2.0-16 ꙡg/kg, OTA 0.15-4.4 ꙡg/kg, T-2 0.5-7.5 ꙡg/kg, HT-2 0.70- 7.5 ꙡg/kg), and ZON 1.3-7.2 ꙡg/kg), depending on matrix. Method performance was verified with reference and quality control materials. Selectivity and confirmation of identity were also demonstrated.

Conclusions: The 11+Myco MS-PREP® IAC with LC-MS/MS method demonstrated acceptable performance for simultaneous determination of 12 mycotoxins in 7 matrixes.

Highlight: The data were reviewed by the AOAC Performance Tested Methods SM Program and approval was granted for certification of the 11+Myco MS-PREP® Method as PTM 112401.