The Utility of Total Thrombus-Formation Analysis System (T-TAS) in the Thrombosis and Hemostasis Field: A Scoping Review

H Mansouritorghabeh; A Monard; F Heubel-Moenen; J Leentjens; A Stroobants; Y Henskens

doi:10.1111/ijlh.14403

The Utility of Total Thrombus-Formation Analysis System (T-TAS) in the Thrombosis and Hemostasis Field: A Scoping Review

Int J Lab Hematol. 2024 Dec 10. doi: 10.1111/ijlh.14403. Online ahead of print.

Authors

H Mansouritorghabeh^{1

2}, A Monard^{3

4}, F Heubel-Moenen³, J Leentjens⁵, A Stroobants⁶, Y Henskens^{4

7}

Affiliations

¹ Central Diagnostic Laboratories, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
² Department of Internal Medicine, Division of Thrombosis and Hemostasis, Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, Leiden, The Netherlands.
³ Department of Internal Medicine - Hematology, Maastricht University Medical Centre+, Maastricht, The Netherlands.
⁴ CARIM-School for Cardiovascular Disease, Maastricht University, Maastricht, The Netherlands.
⁵ Department of Internal Medicine-Vascular Medicine, Radboud University Medical Center, RadboudUMC, Nijmegen, The Netherlands.
⁶ Department of Clinical Chemistry, Radboud University Medical Center, RadboudUMC Laboratory for Diagnostics and Laboratory for Hematology, Nijmegen, The Netherlands.
⁷ Central Diagnostic Laboratory, Unit for Hemostasis and Transfusion, Maastricht University Medical Centre+, Maastricht, The Netherlands.

PMID: 39659111
DOI: 10.1111/ijlh.14403

Abstract

Background: A wide variety of laboratory hemostasis tests is available, but the majority is plasma-based, static and unable to assess platelet function and fibrin formation simultaneously. The Total Thrombus-Formation Analysis System (T-TAS) is a microchip-based flow chamber system that simulates in vivo conditions for evaluating whole blood thrombogenicity.

Aim: A comprehensive overview of its applicability in different thrombosis and hemostasis related clinical situations is lacking and therefore this scoping review was performed.

Materials & methods: A literature search was done using the electronic databases PubMed, Scopus and Embase on January 7, 2024. Original studies assessing the usefulness of the T-TAS in thrombosis and hemostasis related clinical situations were eligible for this scoping review.

Results: A total of 28 studies were included; six studies investigating the role of the T-TAS in congenital bleeding disorders, five studies using the T-TAS to assess 1-year bleeding risk in patients on antiplatelet or anticoagulant medications, four studies investigating the effects of thrombocytopenia and hemodialysis on thrombus formation as measured by the T-TAS, 11 studies testing the applicability of the T-TAS in the monitoring of anticoagulant and antiplatelet therapies and eventually two studies on the ability of the T-TAS to assess the thrombogenicity in different disease entities.

Discussion & conclusion: The T-TAS method is an interesting technology that mimics the complex biological coagulation process using shear forces, creating a "blood vessel component on a chip". More research is needed, but it could eventually function as a screening test for platelet function and coagulation. Moreover, it could be used to detect the presence of anticoagulant and/or antiplatelet medication.

Keywords: Total Thrombus‐Formation Analysis System (T‐TAS); anticoagulants; bleeding disorders; flow chamber; hemophilia; laboratory analysis; platelet; von Willebrand disease; von Willebrand factor.

Publication types

Review