Regulation of states of consciousness by supramammillary nucleus glutamatergic neurones during sevoflurane anaesthesia in mice

Jia-Yi Wu; Wei Wang; Xin-Yi Dai; Si He; Fan-He Song; Shao-Jie Gao; Long-Qing Zhang; Dan-Yang Li; Lin Liu; Dai-Qiang Liu; Ya-Qun Zhou; Pei Zhang; Bo Tian; Wei Mei

doi:10.1016/j.bja.2024.10.023

Regulation of states of consciousness by supramammillary nucleus glutamatergic neurones during sevoflurane anaesthesia in mice

Br J Anaesth. 2024 Dec 6:S0007-0912(24)00663-9. doi: 10.1016/j.bja.2024.10.023. Online ahead of print.

Authors

Jia-Yi Wu¹, Wei Wang², Xin-Yi Dai¹, Si He², Fan-He Song¹, Shao-Jie Gao¹, Long-Qing Zhang¹, Dan-Yang Li¹, Lin Liu¹, Dai-Qiang Liu¹, Ya-Qun Zhou¹, Pei Zhang³, Bo Tian⁴, Wei Mei⁵

Affiliations

¹ Department of Anaesthesiology and Pain Medicine, Hubei Key Laboratory of Geriatric Anaesthesia and Perioperative Brain Health, and Wuhan Clinical Research Center for Geriatric Anaesthesia, Tongji Hospital, China.
² Department of Physiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
³ Department of Neurobiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
⁴ Department of Neurobiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address: tianbo@mails.tjmu.edu.cn.
⁵ Department of Anaesthesiology and Pain Medicine, Hubei Key Laboratory of Geriatric Anaesthesia and Perioperative Brain Health, and Wuhan Clinical Research Center for Geriatric Anaesthesia, Tongji Hospital, China. Electronic address: wmei@hust.edu.cn.

PMID: 39645516
DOI: 10.1016/j.bja.2024.10.023

Abstract

Background: The supramammillary nucleus (SuM), located in the caudal hypothalamus, includes wake-promoting glutamatergic neurones. Their potential role in regulating states of consciousness during general anaesthesia remains unknown.

Methods: We used in vivo fibre photometry, c-Fos staining, chemogenetic and optogenetic manipulations, and electroencephalography/electromyography to explore the roles of glutamatergic SuM neurones (SuM^Vglut2 neurones) at different phases of sevoflurane anaesthesia. Rabies-mediated retrograde and anterograde tract tracing were used to investigate the monosynaptic glutamatergic inputs from the medial septum (MS) to SuM. Their roles in sevoflurane anaesthesia were investigated by in vivo fibre photometry and optogenetic manipulations.

Results: The population activity of SuM^Vglut2 neurones decreased at loss of consciousness but increased during recovery of consciousness under sevoflurane anaesthesia. Their activity also decreased during suppression but increased during bursts in sevoflurane-induced burst-suppression oscillations. Activating SuM^Vglut2 neurones chemogenetically or optogenetically decreased sensitivity to sevoflurane, induced behavioural arousal and cortical activation during continuous steady-state anaesthesia, and stable burst-suppression oscillations under sevoflurane. In contrast, chemogenetic or optogenetic inhibition of SuM^Vglut2 neurones increased sensitivity to sevoflurane or intensified cortical inhibition during sevoflurane anaesthesia. Retrograde and anterograde tracing verified monosynaptic projections from MS^Vglut2 neurones to SuM^Vglut2 neurones. The activity of MS^Vglut2 SuM terminals increased during loss of consciousness but recovered during recovery of consciousness. Optogenetic activation or inhibition of MS^Vglut2 SuM terminals induced cortical activation or inhibition, respectively, during sevoflurane anaesthesia.

Conclusions: Activation of SuM^Vglut2 neurones or the glutamatergic septo-supramammillary circuit induces behavioural arousal and cortical activation during sevoflurane anaesthesia.

Keywords: consciousness; glutamatergic neurones; mechanism of anaesthesia; medial septum; supramammillary nucleus.