CD14loCD301b+ macrophages gathering as a proangiogenic marker in adipose tissues

Yibing Lv; Yidan Zheng; Shanshan Su; Junyi Xiao; Jie Yang; Lingyun Xiong; Yanyan Guo; Xiaoqi Zhou; Nengqiang Guo; Ping Lei

doi:10.1016/j.jlr.2024.100720

CD14^loCD301b⁺ macrophages gathering as a proangiogenic marker in adipose tissues

J Lipid Res. 2024 Dec 5;66(1):100720. doi: 10.1016/j.jlr.2024.100720. Online ahead of print.

Authors

Yibing Lv¹, Yidan Zheng², Shanshan Su³, Junyi Xiao², Jie Yang³, Lingyun Xiong³, Yanyan Guo², Xiaoqi Zhou⁴, Nengqiang Guo⁵, Ping Lei⁶

Affiliations

¹ Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Henan Provincial Key Laboratory of Genetic Diseases and Functional Genomics, Medical Genetic Institute of Henan Province, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, China.
² Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
³ Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
⁴ Department of Transfusion Medicine, Wuhan Hospital of Traditional Chinese and Western Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
⁵ Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address: guonq2012@hotmail.com.
⁶ Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address: adaleip@hust.edu.cn.

Abstract

The role of the monocyte marker CD14 in the regulation of obesity is increasingly recognized. Our observations indicated that Cd14^-/- mice exhibited a leaner body shape compared to their wild-type (WT) counterparts. And the loss of CD14 alleviated high-fat diet-induced obesity in mice. In human subjects, CD14 level was tested to be positively correlated with overweight and obesity. However, the relationship between CD14 and the development of obesity remains only partially understood. To investigate the underlying mechanisms, adipose tissues (ATs) from Cd14^-/- and WT mice were subjected to deep RNA sequencing. Gene Ontology enrichment analysis revealed a significant enhancement of angiogenesis-related function in the Cd14^-/- epididymal adipose tissues compared to WT counterpart, which was accompanied by an upregulation of Cd301b. Subsequent assays confirmed the enhanced angiogenesis and more accumulation of CD301b⁺ macrophages in Cd14^-/- epididymal adipose tissues. Because Igf1 expression has been suggested to be associated with Cd301b expression through pseudotime analysis, we found it was insulin-like growth factor 1 secreted from Cd14^-/- macrophages that mediated the angiogenesis enhancement. Collectively, our ﬁndings indicate that CD14 deficiency increased the accumulation of CD14^loCD301b⁺ macrophages in ATs, which may serve as a proangiogenic marker, providing novel insights into the relationship between CD14 and obesity development.

Keywords: CD14; CD301b; angiogenesis; macrophages; obesity.