The withdrawal time of enrofloxacin, sulfachloropyrazine sodium, and doxycycline as well as the in vitro binding interaction with melanin in black-feathered silky fowl

Yongqin Li; Jiawei Du; Qi Yang; Ruiqian Li; Shuangyuan Jin; Xuelian Guo; Xueyan Wang; Wen Zhang; Lihua Xu

doi:10.1016/j.fochx.2024.101994

The withdrawal time of enrofloxacin, sulfachloropyrazine sodium, and doxycycline as well as the in vitro binding interaction with melanin in black-feathered silky fowl

Food Chem X. 2024 Nov 12:24:101994. doi: 10.1016/j.fochx.2024.101994. eCollection 2024 Dec 30.

Authors

Yongqin Li^{1

2}, Jiawei Du¹, Qi Yang², Ruiqian Li¹, Shuangyuan Jin¹, Xuelian Guo¹, Xueyan Wang¹, Wen Zhang², Lihua Xu¹

Affiliations

¹ College of Animal Science and Technology, Ningxia University, Yinchuan, Ningxia 750021, China.
² Ningxia Institute of Veterinary Durg and Feed Control, Yinchuan, Ningxia 750021, China.

Abstract

The problem of veterinary drug residues in black-feathered silky fowl (BFS) has garnered significant attention. This study investigated the residue elimination patterns and causes of enrofloxacin (ENR), sulfachloropyrazine sodium (SPZ), and doxycycline (DOX) in BFS using ultra-high performance liquid chromatography mass spectrometry (UPLC-MS), along with the drug adsorption and melanin binding tests. The findings indicated that ENR and SPZ were metabolized slowly, DOX was metabolized faster in BFS, with optimum withdrawal time was 87.8, 37.26, and 24.7 days, respectively. The adsorption capacity of skin + fat to drugs and the binding capacity of melanin to the three drugs in BFS were in the order of ENR > SPZ > DOX, confirming that melanin's binding ability influences the drug residues in BFS. This study is highly significant for addressing the issue of veterinary drug residues in BFS.

Keywords: Black-feathered silky fowl; Doxycycline; Enrofloxacin; Mass spectrometry; Melanin; Sulfachlorpyrazine sodium; Withdrawal time.