Automatic detection of multi-antibiotic residues simultaneously in aquatic products by visual protein microarray chips

Abstract

A 96-well microplate-based protein microarray chip which can simultaneously detect three different families of antibiotics using a multiplexed approach has been developed for the first time. All steps in the microarray chip can be completed through an automated biochip analyzer, enabling high throughput analysis of 96 samples within 60 min according to the set program. The microarray chip can be visually evaluated by the color depth of array points, and can also be quantified through a scanner. Under the optimal conditions, the limits of detection (LODs) of nitrofuran metabolites (NFMs), chloramphenicol (CAP), and fluoroquinolones (FQs) were 0.11-0.15 μg/kg, 0.07 μg/kg and 6.42 μg/kg, respectively. This microarray chip can be applied to different aquatic products, including crucian carp, shrimp, and scallops, with the recovery ranged from 89.4 % to 111.2 %. These results demonstrate new strategies for the high-throughput detection of multi-antibiotic residues and present potential of protein chip technologies for daily supervision.

Keywords: Aquatic products; Automatic; Multi-antibiotic; Simultaneous detection; Visual protein microarray chips.