Immunochromatographic Lateral Flow Assays to Detect Filovirus Nucleoproteins

Shino Muramatsu; Daisuke Kato; Ayato Takada

doi:10.1007/978-1-0716-4256-6_29

Immunochromatographic Lateral Flow Assays to Detect Filovirus Nucleoproteins

Methods Mol Biol. 2025:2877:421-430. doi: 10.1007/978-1-0716-4256-6_29.

Authors

Shino Muramatsu¹, Daisuke Kato¹, Ayato Takada^{2

3

4

5}

Affiliations

¹ Denka Co., Ltd., Tokyo, Japan.
² Division of Global Epidemiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Japan. atakada@czc.hokudai.ac.jp.
³ International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Japan. atakada@czc.hokudai.ac.jp.
⁴ One Health Research Center, Hokkaido University, Sapporo, Japan. atakada@czc.hokudai.ac.jp.
⁵ Department of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia. atakada@czc.hokudai.ac.jp.

PMID: 39585638
DOI: 10.1007/978-1-0716-4256-6_29

Abstract

The recent large outbreaks of Ebola virus disease in West Africa and the Democratic Republic of the Congo have highlighted the need for rapid diagnostic tests to control this disease. In this chapter, the development of immunochromatographic lateral flow assays to detect filovirus nucleoproteins is described as an example of designing rapid diagnostic tests.

Keywords: Ebola virus; Filoviral nucleoprotein; Immunochromatography; Lateral flow assay; Latex; Marburg virus; Monoclonal antibody; Nitrocellulose membrane; Rapid diagnostic test.

MeSH terms

Chromatography, Affinity* / methods
Ebolavirus* / immunology
Filoviridae / immunology
Hemorrhagic Fever, Ebola* / diagnosis
Hemorrhagic Fever, Ebola* / immunology
Hemorrhagic Fever, Ebola* / virology
Humans
Immunoassay / methods
Nucleoproteins* / immunology
Nucleoproteins* / metabolism

Substances

Nucleoproteins