Sensitive and accurate detection of thiamethoxam in tea is significant to ensuring consumer health. In this study, surface-enhanced Raman scattering (SERS) tags were prepared by using a polyphenol-mediated coating engineering strategy. This approach involved the self-assembly of tannic acid (TA) and self-polymerization of benzene-1,4-dithiol (BDT) on the surface of gold nanoparticles, resulting in the formation of Au@pBDT-TA. The SERS tags possess high Raman signals and antibody adsorption properties, avoiding the complex decoration or label steps of SERS reporters. We discovered that Au@pBDT-TA, composed of gold nanoparticles at 40 nm and a pBDT-TA thickness of 10 nm, was optimal for the development of the SERS lateral flow immunoassay (LFIA). In comparison to the gold nanoparticle-based LFIA, the SERS-LFIA demonstrated 4-fold and 720-fold enhancements in visual and quantitative limits of detection in buffer solution. The SERS-LFIA demonstrated quantitative limits of detection of 0.06 and 0.1 ng/g for black and green tea, respectively, with a broader linear range spanning over 2 orders of magnitude and a short detection time of 20 min. The proposed SERS-LFIA not only offers a sensitive and reliable method for monitoring thiamethoxam in tea but also possesses a versatile potential that can be easily adapted for the trace detection of various other targets.
Keywords: lateral flow immunoassay; surface-enhanced Raman scattering; tea; thiamethoxam.