Robust testing strategies are an essential aspect of COVID-19 pandemic preparedness and response. In 2022, most regions of Cameroon were still below the WHO's recommended level of 10 COVID-19 tests per 10,000 population. This study aimed to detect SARS-CoV-2 cases in university settings in Cameroon using antigen rapid diagnostic tests (Ag-RDTs) to increase national testing capacity and assess the knowledge, attitudes, and practices of this population regarding COVID-19 infection. Six universities in Buea, Douala, Dschang, Maroua, Ngaoundere, and Yaounde participated in this study from June to October 2022. Nasopharyngeal swabs were collected from participants and tested for COVID-19 using Ag-RDTs. For all positive cases, high-risk contacts were also tested by Ag-RDT. Participants were administered a structured questionnaire to assess their knowledge, attitudes, and practices regarding COVID-19. A total of 7,006 participants were recruited, and 54 (0.8%) were positive for SARS-CoV-2. Among close contacts, three of 62 (4.8%) tested positive. The University of Maroua was the only site to consistently report satisfactory testing capacity, achieving the study target of 30 tests/10,000 in 94.1% of cases. Participants' knowledge of COVID-19 was moderate to good (≥50%). However, 28% were unsure about the effectiveness of the COVID-19 vaccine. Two main factors were identified that could facilitate the spread of SARS-CoV-2 in university settings, namely the lack of restrictions on entering campus without a mask (36%) and the non-respect of social distancing on campus (42.7%). The results of this study will guide future policies to better control diseases with epidemic or pandemic potential by targeting educational institutions.