Sensitive detection of α-amylase based on host-guest inclusion system of γ-cyclodextrin and dansyl-derived diphenylalanine

Spectrochim Acta A Mol Biomol Spectrosc. 2025 Feb 5:326:125291. doi: 10.1016/j.saa.2024.125291. Epub 2024 Oct 16.

Abstract

A highly sensitive detection system for α-amylase was developed via host-guest complexation between γ-cyclodextrin and dansyl-modified diphenylalanine (FF-Dns). The host-guest inclusion of FF-Dns into the cavity of γ-CD in a HEPES buffer solution (10 mM, pH 7.4) significantly enhanced the fluorescence intensity, and the emission wavelength gradually shifted from 558 to 535 nm. The hydrolysis of γ-CD by the addition of α-amylase released FF-Dns, leading to the recovery of the fluorescence emission characteristics. Therefore, the FF-Dns/γ-CD host-guest complexation system can serve as a platform for the sensitive detection of α-amylase with good selectivity against potential interference. The limit of detection (LOD) of the system was 0.004 U/mL, with a linear working range of 0-6 U/mL. The detection assay was successfully applied in 0.1 % serum, achieving an LOD of 0.017 U/mL and a linear working range of 0-10 U/mL.

Keywords: Dansyl; Fluorescent probe; Host–guest recognition; α-Amylase.

MeSH terms

  • Dansyl Compounds* / chemistry
  • Dipeptides* / analysis
  • Dipeptides* / blood
  • Dipeptides* / chemistry
  • Humans
  • Limit of Detection*
  • Phenylalanine* / analogs & derivatives
  • Phenylalanine* / analysis
  • Phenylalanine* / blood
  • Phenylalanine* / chemistry
  • Spectrometry, Fluorescence* / methods
  • alpha-Amylases* / analysis
  • alpha-Amylases* / blood
  • alpha-Amylases* / metabolism
  • gamma-Cyclodextrins* / chemistry

Substances

  • gamma-Cyclodextrins
  • Dipeptides
  • alpha-Amylases
  • Phenylalanine
  • Dansyl Compounds
  • gamma-cyclodextrin
  • phenylalanylphenylalanine