The metabolomic composition of the oviductal fluid is controlled by the periovulatory hormonal context in Bos indicus cows

Angela Gonella-Diaza; Mariana Sponchiado; Moana Rodrigues França; Lihe Liu; Guilherme Pugliesi; Edson Guimarães Lo Turco; Francisco Peñagaricano; Mario Binelli

doi:10.1093/biolre/ioae153

The metabolomic composition of the oviductal fluid is controlled by the periovulatory hormonal context in Bos indicus cows

Biol Reprod. 2024 Oct 17:ioae153. doi: 10.1093/biolre/ioae153. Online ahead of print.

Authors

Angela Gonella-Diaza^{1

2}, Mariana Sponchiado³, Moana Rodrigues França^{2

4}, Lihe Liu⁵, Guilherme Pugliesi², Edson Guimarães Lo Turco⁶, Francisco Peñagaricano⁵, Mario Binelli⁷

Affiliations

¹ North Florida Research and Education Center. Institute of Food and Agricultural Sciences. University of Florida. Marianna, Florida.
² Department Animal Reproduction, University of São Paulo, Pirassununga, São Paulo, Brazil.
³ Division of Animal Sciences, College of Agriculture, Food and Natural Resources, University of Missouri, Columbia, Missouri, USA.
⁴ Campo Real University Center, Guarapuava, Paraná, Brazil.
⁵ Department of Animal and Dairy Sciences, University of Wisconsin-Madison, 1675 Observatory Drive, Madison, WI, 53706, USA.
⁶ Department of Surgery, Division of Urology, Human Reproduction Section, São Paulo Federal University, São Paulo, Brazil.
⁷ Department of Animal Sciences, Institute of Food and Agricultural Sciences. University of Florida. Gainesville, Florida.

PMID: 39418319
DOI: 10.1093/biolre/ioae153

Abstract

In cattle, oviductal function is controlled by the ovarian sex-steroids estradiol and progesterone. Here, we tested the hypothesis that the exposure to contrasting sex-steroid milieus differentially impacts the oviductal fluid composition. Estrous cycles of non-lactating, multiparous Nelore cows were pre-synchronized and then synchronized with a protocol designed two induce ovulation of large (LF group) or small (SF group) follciles. Larger preovulatory follicle (day 0) and corpora lutea (day 4) and greater estradiol (day 0) and progesterone (day 4) concentrations were observed in the LF group. Four days after induced ovulation, oviductal fluid was collected post-mortem. Quantitative mass spectrometry was used to determine the concentration of amino acids, biogenic amines, acylcarnitines, phosphatidylcholines, lysophosphatidylcholines, sphingomyelins, hexoses, prostaglandins and related compounds. Multivariate analyses (OPLS-DA) were conducted to compare the metabolomic signatures of oviductal fluids. Correlation network analysis was conducted to measure the strength and hierarchy of associations among metabolites. Of the 205 metabolites quantified, 171 were detected in at least 50% of the samples and were included in further data analysis. After OPLS-DA analysis, samples of the LF and SF were divided clearly into two non-overlapping clusters. Twenty metabolites had different or tended to have different concentrations in the oviductal fluid when comparing groups. Seven of these 20 analytes had greater concentrations in LF cows. Moreover, total sum of biogenic amines, phosphatidylcholines, and prostaglandins were higher in the SF group. The correlation network showed that the LF group metabolites' concentrations were highly intercorrelated, which was not observed in the SF group. We concluded that the periovulatory endocrine milieu regulates the composition of the oviductal fluid.

Keywords: Amino acids; Cattle; Lipids; Oviduct; Prostaglandins; Sex-steroids.