Fluorescence turn-off detection of myoglobin as a cardiac biomarker using water-stable L-glutamic acid functionalized cesium lead bromide perovskite quantum dots

Mikrochim Acta. 2024 Oct 16;191(11):674. doi: 10.1007/s00604-024-06752-z.

Abstract

Water dispersible L-glutamic acid (Glu) functionalized cesium lead bromide perovskite quantum dots (CsPbBr3 PQDs), namely CsPbBr3@Glu PQDs were synthesized and used for the fluorescence "turn-off" detection of myoglobin (Myo). The as-prepared CsPbBr3@Glu PQDs exhibited an exceptional photoluminescence quantum yield of 25% and displayed emission peak at 520 nm when excited at 380 nm. Interestingly, the fluorescence "turn-off" analytical approach was designed to detect Myo using CsPbBr3@Glu PQDs as a simple optical probe. The developed probe exhibited a wide linear range (0.1-25 µM) and a detection limit of 42.42 nM for Myo sensing. The CsPbBr3@Glu PQDs-based optical probe provides high ability to determine Myo in serum and plasma samples.

Keywords: Biofluids; Cesium lead bromide; Fluorescence; Myoglobin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / analysis
  • Biomarkers / blood
  • Calcium Compounds* / chemistry
  • Cesium* / chemistry
  • Fluorescent Dyes / chemistry
  • Glutamic Acid* / analysis
  • Glutamic Acid* / blood
  • Glutamic Acid* / chemistry
  • Humans
  • Lead* / analysis
  • Lead* / blood
  • Lead* / chemistry
  • Limit of Detection*
  • Myoglobin* / analysis
  • Myoglobin* / blood
  • Myoglobin* / chemistry
  • Oxides* / chemistry
  • Quantum Dots* / chemistry
  • Spectrometry, Fluorescence / methods
  • Titanium / chemistry
  • Water / chemistry

Substances

  • Lead
  • Myoglobin
  • Glutamic Acid
  • Calcium Compounds
  • Cesium
  • Oxides
  • Titanium
  • Biomarkers
  • lead bromide
  • Fluorescent Dyes
  • perovskite
  • Water