Thioclava arctica sp. nov. and Zhongshania arctica sp. nov., isolated from the surface sediment of the Arctic Ocean and reclassification of Marortus luteolus Yu et al. 2019 as a later heterotypic synonym of Zhongshania marina On et al. 2019

Panyi Pan; Jianning Wang; Xiupian Liu; Qiliang Lai; Guangyu Li; Yaping Du; Jinzhu Zhang; Ziyuan He; Zongze Shao; Chunming Dong

doi:10.1099/ijsem.0.006548

Thioclava arctica sp. nov. and Zhongshania arctica sp. nov., isolated from the surface sediment of the Arctic Ocean and reclassification of Marortus luteolus Yu et al. 2019 as a later heterotypic synonym of Zhongshania marina On et al. 2019

Int J Syst Evol Microbiol. 2024 Oct;74(10). doi: 10.1099/ijsem.0.006548.

Authors

Panyi Pan^{1

2}, Jianning Wang², Xiupian Liu², Qiliang Lai^{1

2

3}, Guangyu Li², Yaping Du², Jinzhu Zhang^{1

2}, Ziyuan He^{2

4}, Zongze Shao^{1

2}, Chunming Dong^{1

2}

Affiliations

¹ School of Advanced Manufacturing, Fuzhou University, Jinjiang 362251, PR China.
² Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources of China; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.
³ Xiamen Ocean Vocational College, Xiamen 361005, PR China.
⁴ School of Marine Sciences, China University of Geosciences, Beijing 100083, PR China.

PMID: 39412521
DOI: 10.1099/ijsem.0.006548

Abstract

Two Gram-stain-negative bacterial strains, 15-R06ZXC-3^T and R06B22^T, were isolated from the surface sediment of the Arctic Ocean. Phylogenetic analyses based on the 16S rRNA gene and genome sequences indicated that strain 15-R06ZXC-3^T belongs to the genus Thioclava, while strain R06B22^T belongs to the genus Zhongshania. Strain 15-R06ZXC-3^T showed the closest relationship to Thioclava indica DT23-4^T. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain 15-R06ZXC-3^T and all of the type strains of the genus Thioclava ranged from 20.8 to 30.4% and 79.1 to 85.7%, respectively. Strain R06B22^T was most closely related to Zhongshania marina DSW25-10^T. The dDDH and ANI values between strain R06B22^T and all of the type strains of the genus Zhongshania ranged from 18.6 to 20.4% and 77.5 to 79.4%, respectively. These dDDH and ANI values were all below the standard cutoff criteria for the delineation of bacterial species, indicating that the two strains may represent novel species within their respective genera. Furthermore, their phenotypic and chemotaxonomic characteristics also differentiated them from closely related species. Based on the polyphasic analyses, strains 15-R06ZXC-3^T and R06B22^T separately represent novel species of the genera Thioclava and Zhongshania, for which the names Thioclava arctica sp. nov. (type strain 15-R06ZXC-3^T = MCCC 1A07434^T= KCTC 8342^T) and Zhongshania arctica sp. nov. (type strain R06B22^T = MCCC 1A08273^T= KCTC 8343^T) are proposed. Additionally, phylogenomic analyses showed that the strain Marortus luteolus ZX-21^T was clustered with the strain Z. marina DSW25-10^T and all other type strains of the genus Zhongshania. Furthermore, the ANI and dDDH values between strains ZX-21^T and DSW25-10^T were 97.6% and 78.8±2.5%, respectively, strongly indicating that they represented a single species. Therefore, it is proposed that M. luteolus Yu et al. 2019 be recognized as a later heterotypic synonym of Z. marina On et al. 2019.

Keywords: Arctic Ocean; Chukchi Sea; Thioclava arctica sp. nov.; Zhongshania arctica sp. nov.; polyphasic taxonomy; surface sediment.

MeSH terms

Arctic Regions
Bacterial Typing Techniques*
Base Composition
DNA, Bacterial* / genetics
Fatty Acids / analysis
Fatty Acids / chemistry
Geologic Sediments* / microbiology
Nucleic Acid Hybridization*
Oceans and Seas
Phylogeny*
RNA, Ribosomal, 16S* / genetics
Seawater / microbiology
Sequence Analysis, DNA*

Substances

RNA, Ribosomal, 16S
DNA, Bacterial
Fatty Acids