Genome-wide identification and expression analysis of SlKFB gene family (Solanum lycopersicum) and the molecular mechanism of SlKFB16 and SlKFB34 under drought

Lei Yu; Xiaoyu Guan; Fanyue Meng; Fulei Mo; Rui Lv; Zhen Ding; Peiwen Wang; Xiuling Chen; Mozhen Cheng; Aoxue Wang

doi:10.1016/j.plaphy.2024.109192

Genome-wide identification and expression analysis of SlKFB gene family (Solanum lycopersicum) and the molecular mechanism of SlKFB16 and SlKFB34 under drought

Plant Physiol Biochem. 2024 Oct 10:216:109192. doi: 10.1016/j.plaphy.2024.109192. Online ahead of print.

Authors

Lei Yu¹, Xiaoyu Guan², Fanyue Meng³, Fulei Mo⁴, Rui Lv⁵, Zhen Ding⁶, Peiwen Wang⁷, Xiuling Chen⁸, Mozhen Cheng⁹, Aoxue Wang¹⁰

Affiliations

¹ College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, China. Electronic address: yuleineau@163.com.
² College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, China. Electronic address: guanxiaoyu0924@126.com.
³ College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, China. Electronic address: mengfanyue1@126.com.
⁴ College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, China. Electronic address: neaumfl@163.com.
⁵ College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, China. Electronic address: lvrui34324080@163.com.
⁶ College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, China. Electronic address: 13555091770@163.com.
⁷ College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, China. Electronic address: wangpeiwen@neau.edu.cn.
⁸ College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, China. Electronic address: chenx@neau.edu.cn.
⁹ College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, China. Electronic address: mzcheng@neau.edu.cn.
¹⁰ College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, China. Electronic address: axwang@neau.edu.cn.

PMID: 39406005
DOI: 10.1016/j.plaphy.2024.109192

Abstract

Environmental stress significantly affects plant growth and productivity. The effects of drought stress on plants are reflected primarily in enzyme activity, membrane systems, and cell-water loss. Here, the Kelch repeat F-box (KFB) protein family in tomato was systematically identified and analysed. Using bioinformatics, we identified 37 SlKFB family members in the tomato genome and analysed their protein structure, phylogenetic relationships, chromosome distribution, and expression under drought or biotic-stress conditions. Transcriptome data revealed that SlKFB members exhibit differential responses to drought stress, with significant differences in SlKFB16 and SlKFB34 expression. Functional analysis revealed that SlKFB16 functions in the cytoplasm and SlKFB34 in the nucleus and cytoplasm. Under drought stress, SlKFB16 and SlKFB34-silencing significantly reduced reactive oxygen species scavenging and resistance to drought stress. These findings provide a reference for further studies of the mechanisms of SlKFB16 and SlKFB34 in drought stress in tomato as well as a foundation for enhancing their resistance to drought stress.

Keywords: Drought stress; Kelch repeat F-Box; Tomato; VIGS.